Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
Wash Tips Command
Use Waste
Specify the worktable position for the waste you want to use by clicking on it in
the Worktable Editor. You must first put a wash station with waste unit in the
Worktable Editor at the required position. Alternatively, choose the required
waste position from the pull down list. The list shows all waste units which are
currently positioned in the Worktable Editor.
The gray (protected) fields show the worktable coordinates of the selected
waste.
Use Cleaner
Specify the worktable position for the cleaner you want to use by clicking on it
in the Worktable Editor. You must first put a wash station with cleaner unit in
the Worktable Editor at the required position. Alternatively, choose the
required cleaner position from the pull down list. The list shows all cleaner
units which are currently positioned in the Worktable Editor.
The gray (protected) fields show the worktable coordinates of the selected
cleaner.
Choose a shallow cleaner if you only need to clean the ends of the tips.
Choose a deep cleaner if there is a possibility of contamination along the shaft
of the tip. The deep cleaner requires a larger volume of system liquid and
cleaning takes somewhat longer.
The wash cycle is skipped if you are flushing DITI adapters and Use Cleaner
is ignored in this case.
Use Fast-Wash Module
If your instrument is fitted with FWO (Fast Wash Option) or MPO (Monitored
Pump Option), check this checkbox to use the Fast Wash module instead of
the dilutors to wash the tips. If you use the Fast Wash module, the volumes of
liquid which are dispensed in the flushing and washing steps may differ
significantly from the volumes you specify. This is due to accuracy limitations
of the pump.
Use Pinch Valve
If your instrument is fitted with the Low Volume Option, check this checkbox to
activate the Low Volume Option after washing the tips.
Delay in Waste
To reduce the pressure inside the tubing, you can instruct the instrument to
keep the valves open somewhat longer after flushing before moving the LiHa
away from the waste (range: 0 - 1000 ms).
Delay in Cleaner
To reduce the pressure inside the tubing, you can instruct the instrument to
keep the valves open somewhat longer after washing before moving the LiHa
away from the cleaner (range: 0 - 1000 ms). The wash cycle is skipped if you
are flushing DITI adapters and Delay in Cleaner is ignored in this case.
Retract with
Specify the speed with which you want to retract the tips from the cleaner
(range: 0 - 100 mm/s). If the speed is too high, droplets may be dragged out of
the cleaner and stick to the outside of the tips.
Airgap volume, Airgap speed
These settings are used to specify a system trailing airgap (STAG) which will
be aspirated after washing the tips. Specify the required size of the airgap in µl
and the aspiration speed for the airgap in µl/s. The system trailing airgap is
used to prevent contamination by ensuring that the system liquid cannot come
into contact with the liquid which will be aspirated in a subsequent pipetting
command.
Freedom EVOware Software Manual, 393172, en, V2.3 15 - 9