Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
Replicates Wizard
– With 12 to 1122, Freedom EVOware pipettes all replicates of the first
source column onto the destination plate, and then proceeds with the
replicates of the second source column. For example, if you want to
replicate columns B, C and D of the source labware and you choose three
replications per plate, the destination columns will be as follows:
BBBCCCDDD.
– With 12 to 1212, Freedom EVOware pipettes a single replicate of the first
column and then a single replicate of the second column, and so on. For
example, if you want to replicate columns B, C and D of the source plate
and you choose three replications per plate, the destination columns will
be as follows: BCDBCDBCD.
Volume in destination wells
Specify the required volume of liquid in the destination wells (the same volume
will be dispensed to all wells of all replicates). Freedom EVOware
automatically calculates the necessary aspiration volume for the source
labware. However, Freedom EVOware does not automatically handle
volumes that exceed the dilutor or DITI capacity.
15.29.3 Replicates Wizard, Step 3 of 5
Select the destination plate parameters:
Fig. 15-36 Replicates wizard, step 3 of 5
Do you want to mix after dispensing
Click the appropriate radio button. If you choose Yes, you must also specify
how many times to mix and the volume to mix with.
Wash tips after x replications
This option only appears if you are using fixed tips. Enter a non-zero value if
you want to wash the tips after replicating. If you specify “1”, washing will be
carried out after each replication.
15 - 66 Freedom EVOware Software Manual, 393172, en, V2.3