Збірник тез XVI Міжнародно медичного конгресу студентів та ...

Eng Lu Sun, Emerodi Chidera, Kenechukwu Rose
STUNNING AND HYBERNATING MYOCARDIUM OF NEWBORN
Departments of human anatomy and hospital‘s pediatry
Scientific leaders: d.m.s., professor Igor V. Zadnipryany, d.m.s., professor Olga S. Tretiakova
SO ―Crimea medical university after S. I. Georgievsky‖
Simferopol, Ukraine
Recent research found that the effect of perinatal hypoxia leads to ischemia of the heart muscle newborns,
which is implemented by decreased myocardial contractility, arrhythmias, conduction, and the development of
cardiovascular disease, which in severe cases, determines the fatal disease. Ischemia and reperfusion of the
myocardium leads to severe irregularities in energy production and damage to cellular structures of
cardiomyocytes, particularly in the restoration of supply cells with oxygen. The aim of the study was the
identification of morphological substrate of hypoxic cardiac damage at perinatal hypoxia.
The group surveyed included 22 infants early neonatal period, who were treated in neonatal intensive care
units with a diagnosis of perinatal injury to the central nervous system hypoxic-ischemic (or / trauma) origin. An
experimental study was carried out on 8 rats of Wistar females and their 14 newborn rats. In order to detect
microscopic signs of myocardial hypoxia were applied conventional histological techniques (hematoxylin-eosin,
Van Gisone, Lee). To identify the ultrastructural signs of damage to the heart muscle in 5 cases (including
myocardial cell material newborns - 5 cases of rat hearts. Enzymological activity of creatine kinase MB-kinetic
method, evaluated erythrocyte levels of arachidonic acid, diene and triene by the method of conjugate, the level of
lipid hydroperoxide, malonic aldehyde - method.
In the heart of neonatal hypoxic injury develops the phenomenon of "stunned" or "re-perfused" infarction,
baseline which is the progressive damage of intracellular structures, due to the formation of large amounts of free
radicals that cause the heart muscle dis-metabolism and lead ultimately to a "cell stroke." The development of the
phenomenon of "stunned" myocardium requires a pathogenically substantiated metabolic therapy aimed at
stabilizing the membranes of cardiomyocytes, optimization of energy metabolism of ischemic myocardium.
Lamptey William
MANTIAL GLIOCYTES CHANGES IN EARLY STAGE AFTER
EXPERIMENTAL BURN OF SKIN
Department of histology, cytology and embryology
Supervisor of studies: doc. A.P. Andriyishyn
Ternopil State Medical University
named after I.J. Horbachevsky
Ternopil, Ukraine
Thermal injuries of skin are widespread in the world. Burn schock causes significant influence on nervous
system. Sensory neurons are first cells which are affected in such cases. So, histological research of structural
components of dorsal root ganglia may explain some mechanisms of burn disease development .
Dorsal root ganglia were researched in experiment on guinea pigs with burns of IIIA-IIIB degree of 20 % of
skin at the 1 st day after thermal trauma. Histological specimens for ultrastructural research were made in usual way
and observed in electron microscope.
Spinal ganglia consist of pseudounipolar cells which are placed on dorsall root of spinal cord. Each neuron
is surrounded with flat glial cells (oligodendrocytes) – mantial or satellite cells. Nuclear portion of each cell is well
distinct, organelles are placed arround nucleus. Peripheral portion simetime is very thin, but it is present
everywhere arround neuron.
At the stage of burn schock structural changes of satellite cells are characterized by significant enlightening
of cytoplasm. Thickness of mantial cells increases too. Their nuclei yet contain irregularly condensed
heterochromatin, nuclear envelope is irregular, perinuclear space is wider, more nuclear pores are seen. Nucleoli
are rare found in such nuclei Mitochondria have oval shape, matrix is lighter, cristae reduce. Elements of
endoplasmic reticulum are uniformly dispersed arround nuclei, vacuolized, lisosomes are often seen near
dictiosomes of Golgi apparatus. Cisternae and vesicles of latter one are expanded. Peripheral portions of cells are
thicker as in normal condition, cytoplasm here is lighter too.
The obtained data shows reversible changes of mantial cells which are typical for adaptative processes.
This allow to testify experimental thermal burn of skin to cause prominent (considerable) influence on mantial cells
of spinal ganglia.
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