Збірник тез XVI Міжнародно медичного конгресу студентів та ...

Khodabakhshi Ardali Zahra A. Kh.
MOLECULAR-BIOLOGICAL STUDY OF THE MECHANISM OF ACTION OF 2-ETHYL-6-METHYL-3-
OXYPYRIDINE SUCCINATE
Chair of Experimental and Clinical Pharmacology
Scientific mentor: O. Vazhnicha, MD, professor
HSEE of Ukraine ―Ukrainian Medical Stomatological Academy‖
Poltava, Ukraine
It is known, that 2-ethyl-6-methyl-3-oxypyridine succinate (mexidol) is potent synthetic antioxidant. The
drug is 3-oxypyridine derivative containing succinate. It protects lipids of cell membranes against lipid peroxidation
and has antihypoxic properties, anxiolytic, nootropic and neuroprotective effects. However a question about
molecular-biological mechanism of mexidol‘s action on the cell, for example red blood cell (RBC), under the
pathological conditions remains not decided. Research purpose is to study the influence of 2-ethyl-6-methyl-3-
oxypyridine succinate (mexidol) on RBC‘s cytoskeleton and membrane asymmetry. RBC were obtained from intact
albino male rats by the standard procedure of heart punction under the general anesthesia. Mexidol was added to
test-systems in a concentration corresponded to the dose of 100 mg/kg in experiments upon animals. A design of
experiment includes the incubation of RBC in 0,45% solution of sodium chloride with mexidol or without the drug
(control) at 37 o C for 2 hours. The expression of phosphatidylserine on the external surface of cell membrane was
detected by the cytofluorimetric method. Proteins of RBC stroma were purified and undergone electrophoresis,
densitometry and quantitative analysis. It is shown that mexidol decreases the count of RBC with altered
membrane phospholipid asymmetry as compared to control. Electrophoregram of proteins of RBC cytoskeleton is
characterized by the formation of typical bands both in the control and in the samples with investigated preparation.
But densitometry demonstrates some distinguishes between these groups concerning spectrin-alpha, spectrinbeta,
and ankyrin. The data obtained shows that under the conditions of hypoosmolarity mexidol eliminates the
disorders of RBC membrane‘s asymmetry and modifies spectrin-actin complex and vertical membranecytoskeleton
bonds mediated by ankyrin.
Tayel Alaa
THE INFLUENCE OF COMBINED ANTIOXIDANT PREPARATION ON THE AUTONOMIC NERVE
REGULATION IN ELDERLY PATIENTS DURING THEIR ADAPTATION TO COMPLETE DENTURES
Chair of Experimental and Clinical Pharmacology;
Chair of Orthopedic Dentistry and Implantology
Scientific mentors: O.Vazhnicha, MD, professor*; L. Lugova. PhD, lecturer
HSEE of Ukraine ―Ukrainian Medical Stomatological Academy‖
Poltava, Ukraine
Antioxidants are known for their stress-protective properties and are widely applied in gerontology. It
became the precondition for use of combined preparation "Aevit" in patients which get used to dentures as this
process is not especially dental, but is characterized by the signs of common adaptation syndrome and develops
on background of disadaptation caused by ageing of the organism. The research aim is to study the influence of
combined antioxidant preparation ―Aevit‖ on some somatic parameters and Kerdo‘s index during patients‘
adaptation to artificial teeth. Under our supervision there were 32 adentulous patients of elderly and senile age.
Complete removable dentures have been made of dental plastic according to standard technology. One group of
patients served as a control. Patients of other group were prescribed "Аevit" (1 capsule daily during 45 days).
Frequency of heart beats, frequency of breathing, and blood pressure were determined. Kerdo‘s index was
calculated. These parameters were registered before the treatment, and also on the 1 st , the 7 th and the 30 th day
after the start of dentures use. It is shown that patients prescribed with ―Aevit‘ display the dynamics of somatic
parameters which differs from those in patients of control group by the greater expressiveness of changes in the
frequency of heart beats and breath at the end of research. Distinguishes in changes of Kerdo‘s index also are
found out also: its magnitude grew by the module on the 1 st and the 7 th day and return to the initial level in 30 days,
but these fluctuations were smaller than the changes of autonomic regulation index in the control. The found out
features allow to conclude that the development of adaptation in orthopedic dental patients on a background of the
treatment with a combined antioxidant preparation ―Aevit‖ is characterized by the greater stability of autonomic
regulation, especially those processes which concern cardiovascular system and consumption of oxygen.
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