Збірник тез XVI Міжнародно медичного конгресу студентів та ...

Maksimchuk Natalia, Ungurian Andrey
THE STATE OF THE APUD-SYSTEM AND STRUCTURAL PECULIARITIES OF THE INTESTINES DURING
PURULENT OR SEPTIC DISEASES IN AN EXPERIMENT
Department of Pediatric Surgery and Otolaryngology
Cand. Sci. (Med.) Boris Bodnar
Bukovinian State Medical University
Structural changes in the intestines which occur during inflammatory diseases of the abdomen have
important diagnostic and prognostic value which predetermines the importance of studying them. However, despite
the existence of this group of diseases, a significant proportion of unanswered questions remains which have
extremely important clinical significance. One of these issues is the status of the APUD-system of the intestines
that is known to be the leading system of regulation of local homeostasis, thus influencing the course of the
process of inflammation, regeneration, secretion, and motor activity. The above mention role of the APUD-system
can not be overemphasized, because the effects on such physiological processes determines the patient's
recovery and restoration of bowel motility.
Our purpose in this study was to examine the state of the APUD-system of the intestine after past purulentinflammatory
diseases of the abdominal cavity in an experiment. The study was conducted on 75 white rats.
Peritonitis was modeled by introducing the rat's own feces into the abdominal cavity. Collection of material for
histological analysis was performed using standard procedures. In the model of fecal peritonitis, destructive
inflammatory changes were observed in the intestinal wall, which manifestated as the thickening of the serous
membraine and its focal fibrosis, moderate diffuse infiltration of lymphocytes and macrophages. In the study of the
APUD-system a marked reduction in cell number was observed as well as the presence of apudocytes only in
some glands, and a small amount of argentaffin granules in cells. When staining the walls of the intestine by the
Masson-Hamperl method a large amount of APUD-cells were found in glands, when compared with the main
group, for which ozonized physiological solution was not used for sanitation. The amount of apudocytes was
significant in almost all colonic crypts.
The amount of granules and their sizes were larger, granules were located in both basal and apical parts of
cells. This study showed that during peritonitis, dystrophic changes are pronounced, fibrosis of the serous and
muscle membrane of the intestine, reduction in the number and functional activity of APUD-cells that enhance the
process of comissure formation. Morphological study of the effect of ozone on comissure formation showed high
efficiency of this method in preventing the formation of comissures.
Kirichenko Oleg
THE USE OF OZONE THERAPY IN THE POSTOPERATIVE TREATMENT OF ACUTE INTESTINAL
OBSTRUCTION
Department of General Surgery
Dr. (Medicine) and Professor of Science, V. Ganji
Zaporozhye State Medical University
Zaporozhye, Ukraine
Over the past 40 years, the frequency of acute colonic obstruction (ACO) in the occurrence of all forms of
acute intestinal obstruction (AIO) increased from 2-5 % to 20-24%. This pathology is one of the challenges in
terms of diagnosis and treatment, which is characterized by the development of severe complications and high
postoperative mortality. The reasons for ACO are tumors of the colon, extraorganic tumor inversions, sigmoid and
cecum, strangulated hernia, coprostasia, strictures of the large intestine, etc. In spite of recent advances in
abdominal surgery and postoperative intensive care, outcomes in patients with ACO cannot be considered
satisfactory since case fatality rates are 17-21%. Issues of post-operative treatment of ACO continue to be relevant
at the present time, despite some advances in surgical techniques, and the use of modern methods of
detoxification and antibiotic therapy.
In this context it is noteworthy that the universal antimicrobial and biostimulation effect of ozone and
sodium hypochlorite is therapeutic, and can improve post- surgical outcomes. It has been found that ozone in
ozonated solutions of sodium hypochlorite is particularly effective due to high biological activity, as well as a
providing a wide range of therapeutic effects with a minimal risk of subsequent development of inflammatory
complications. The monitoring group in this study was represented by 24 patients, who in addition to traditional
antibacterial and anti-inflammatory therapy in the postoperative period, received ozone in the form of intravenous
infusions of 400 ml of ozonated saline solution with a concentration of ozone at 1500 ug / l. Treatment consisted of
6 sessions, held daily. The control group were patients treated with conventional postoperative therapy. For this
clinical trial, the pre-conditions of members of the selected groups were homogeneous.
Conclusions: The use of intravenous ozone therapy in the complex treatment of patients with postoperative
ACO leads to a positive clinical effect in 88% of cases, significantly reducing the rates of inflammatory syndrome,
significantly increasing the activity of the antioxidant system, and reducing the activation of lipid peroxidation by
21%.
64