AMMONIUM SULFATE CAS N°: 7783-20-2
AMMONIUM SULFATE CAS N°: 7783-20-2
AMMONIUM SULFATE CAS N°: 7783-20-2
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OECD SIDS<br />
<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />
4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />
DATE: 18.04.<strong>20</strong>06<br />
Type:<br />
flow through<br />
Species:<br />
other aquatic worm: Dugesia tigrina<br />
Exposure period: 96 hour(s)<br />
Unit: mg/l Analytical monitoring: no<br />
LC50 : > 100<br />
Method:<br />
GLP:<br />
Test substance:<br />
Remark:<br />
Result:<br />
Test condition:<br />
other: see Test Conditions<br />
no data<br />
other TS: ammonium sulphate, reagent-grade, no further details<br />
In this study, 27 commercial inorganic and organic chemicals<br />
were tested simultaneously in seven aquatic species.<br />
A LC50 value could not be determined because the proportion<br />
killed at the highest dose level (100 mg/L) was less than<br />
50%. No further details reported.<br />
TEST ORGANISMS: Flatworm (Dugesia tigrina, Platyhelminthes),<br />
0.006 g. The test organisms were acclimated to the control<br />
diluent water in the breeding/rearing tanks (time period not<br />
reported). Food was withheld for the 24 hours preceding start<br />
of the test. Juveniles as uniform in size as possible were<br />
collected from the colonies. Ten juvenile organisms were<br />
exposed to the test chemical in each treatment.<br />
Biological loading was kept below 0.5 gram wet weight per<br />
liter of test solution.<br />
TEST VESSEL: The assay was performed in seamless glass,<br />
30.5-cm cuboidal, Pyrex chromatographic jars to which <strong>20</strong><br />
liters of test solution was added. The test organisms were<br />
segregated in welded stainless steel, 55-mesh wirecloth<br />
baskets (5.5 cm in diameter x 7.5 cm in depth). Each basket<br />
was suspended from a 1-rpm motor-driven mechanism that<br />
raised and lowered the baskets in the water column. A<br />
stainless steel band, slotted every 0.5 cm, facilitated the<br />
positioning of the baskets so that the submerged volumes<br />
changed from one-third to wo-thirds during each cycle.<br />
One-half of the volume of the sumerged basket was exchanged<br />
with the main tank volume every minute.<br />
TEST SOLUTION: The test chemical was added directly to the<br />
diluent water to give a nominal concentration of 100 mg/L.<br />
The test chemical concentrations were not analyzed. Once the<br />
test solutions were prepared, the starting temperature,<br />
dissolved oxygen and pH values were determined for each<br />
exposure concentration. When the starting pH of the test<br />
soluton fell outside the extremes of 6.5 to 8.5, the pH was<br />
adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%<br />
(v/v) H2SO4.<br />
DILUTION WATER: Water quality was routinely monitored to<br />
characterize the diluent water and ensure its suitability<br />
according to the standard method for the examination of<br />
water and wastewater, 16th ed., American Public Health<br />
Association, Washington DC pp. 689-832. The chemical<br />
characteristics are reported in the publication and are in<br />
accordance with standards required by current testing<br />
guidelines. Activated carbon-filtered, dechlorinated and<br />
tempered industrial service water from Lake Ontario was used<br />
in all tests.<br />
102<br />
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