AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Reliability:
to give a 12 hours day period and 12 hours darkness.
TEST TEMPERATURE: 23.5 °C, thermostated experimental room.
DILUTION WATER: the dilution water was prepared accodring to
the ISO proposal by dissolving the chemicals in deionized
water. The water was prepared one day before the use and was
kept in PVC tanks.
PILOT TEST: the test was preceded by a least one pilot test
under static conditions. 5 fishes were exposed in each test
jar, and six concentrations were tested.
CONTINUOS-FLOW TEST: the dilution water and the test
substance were distributed by aid of peristaltic pumps
through silicone rubber tubes to a mixing chamber (250 mL
suction flask). the tubes ended at the bottom of the mixing
chamber. Mixing was made by a magnetic stirrer. From the
spout of the mixing chamber, the mixed solution was
delivered to the test jars via silicone rubber tubes. The
test jars consisted of 1 L spherical glass flasks with threen
necks. The test water flow was approximately 1 L/hour during
all the experiments. Six concentrations, including one
control, were used in the tests. Ten fishes were exposed to
each concentration. The condition of the fishes was checked
after 3, 6, 12, 24, 48, 72 and 96 hours. At the same time, the
dissoved oxygen content, pH and temperture were recorded.
(2) valid with restrictions
limited documentation
Flag:
Critical study for SIDS endpoint
23-JAN-2004 (50)
Type:
semistatic
Species:
Brachydanio rerio (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: = 420
Limit Test: no
Method:
GLP:
Test substance:
other: see Test Conditon
no data
other TS: ammonium sulfate, analytical grade
Remark: The LC50 for NH4E+ was quoted to be 114, 130 and 141
mg/L after 96, 48 and 24 hours, respectively. The LC50
values for (NH4)2SO4 after 24 and 48 hours were 520 and 480
mg/L, respectively.
Test condition: Test Temperature: 23.5 °C, thermostated experimental room.
Test Jars: 10 L glass jars with 4 L of test substance. Every
24 hours the fishes were transferred, using a dip net, to
jars with new test solutions. the test water was not aerated
during the tests.
Reliability: (2) valid with restrictions
limited documentation
Flag:
Critical study for SIDS endpoint
23-JAN-2004 (50)
Type:
flow through
Species:
Salmo gairdneri (Fish, estuary, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: yes
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