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AMMONIUM SULFATE CAS N°: 7783-20-2

AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

AQUATIC ORGANISMS<br />

4.1 Acute/Prolonged Toxicity to Fish<br />

Type:<br />

static<br />

Species:<br />

Leuciscus idus (Fish, fresh water)<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: no<br />

LC0: 464<br />

LC50: 681<br />

LC100: 1000<br />

Limit Test: no<br />

Method: other: equivalent to OECD <strong>20</strong>3<br />

Year: 1989<br />

GLP:<br />

yes<br />

Test substance: as prescribed by 1.1 - 1.4<br />

Result:<br />

Clinical signs included apathy and tumbling at 1000 mg/L. No<br />

signs of toxicity were noted at 100, 215, and 464 mg/L.<br />

Mortalities at 96 hours:<br />

100 mg/L: 0/10<br />

215 mg/L: 0/10<br />

464 mg/L: 0/10<br />

1000 mg/L: 10/10<br />

pH values after 1 hour were: 7.6, 7.5, 7.5, 7.3, 7.3 for<br />

control, 100, 215, 464, and 1000 mg/L, respectively.<br />

pH values after 96 hours were: 7.8, 7.7, 7.7, 7.6, and not<br />

determined for control, 100, 215, 464, and 1000 mg/L,<br />

respectively.<br />

Oxygen content (mg/L) after 1 hour was: 7.7, 8.3, 8.5, 8.5,<br />

8.6 for control, 100, 215, 464, and 1000 mg/L, respectively.<br />

Test condition:<br />

Oxygen content (mg/L) after 96 hours was: 8.0, 8.1, 8.2,<br />

8.0, and not determined for control, 100, 215, 464, and 1000<br />

mg/L, respectively.<br />

TEST ORGANISMS: Golden orfe (Leuciscus idus L., golden<br />

variety), 2.9 g (2.1-3.6 g), 6.7 cm (6.1-7.2 cm). The test<br />

organisms were acclimated to the control diluent water in<br />

the breeding/rearing tanks for 3 days. Food was withheld for<br />

the 24 hours preceding start of the test and during the<br />

test. Ten fish were exposed to the test chemical in each<br />

treatment. Biological loading was 2.9 gram wet weight per<br />

liter of test solution.<br />

TEST VESSEL: The tests were performed in all-glass aquaria<br />

of 30cmx22cmx24cm size to which 10 liters of test water were<br />

added.<br />

TEST SOLUTION: The test chemical was added directly to the<br />

diluent water to give nominal concentrations of 100, 215,<br />

464 and 1000 mg/L. The test chemical concentrations were not<br />

analyzed. Once the test solutions were prepared, the<br />

starting temperature, dissolved oxygen and pH values were<br />

determined for each exposure concentration.<br />

When the starting pH of the test soluton fell outside the<br />

UNEP PUBLICATIONS 77

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