AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
sulfate solution prepared in tap water (dissolved oxygen 6
mg/L, water temperature 22 +/- 2 °C, pH 7.8, hardness 23.2
mg/L). In controls, no ammonium sulfate was added. After
every 24 hours the media were renewed. Dead fish, if any,
were immediately removed. Decrease in concentration of
ammonium sulfate between two renewals was not measured.
Fragments of the air sac from the anterior end of 2
experimental and 2 control fish wer fixed in 10% neutral
formalin and Bouin´s fluid from each of the 6 h, 12 h, 1 d,
2 d, 3 d, 4 d, 6 d, 8 d, and 10 d treated specimens. Six
micron thick paraffin sections as well as whole mounts were
stained with Ehrlich´s hematoxylin/eosin, and with various
histochemical methods for different carbohydrate moieties.
Reliability: (2) valid with restrictions
limited documentation
23-JAN-2004 (63)
Type:
semistatic
Species:
other: Heteropneustes fossilis
Exposure period: 45 day(s)
Unit: mg/l Analytical monitoring: no data
LOEC : = 200
Method:
GLP:
Test substance:
Result:
Test condition:
other: see Test Condition
no data
other TS: ammonium sulfate, not further specified
Density and dimension of the goblet mucous cells of the
outer opercular epidermis increased markedly in the initial
stages of exposure. Perinuclear vacuoles appeared in the
necrotic epithelial cells which also bear pyknotic nuclei
before their shedding at several stages of treatment. The
club cells also exhibited great vacuolization. The damage
became more extensive in later stages of exposure when
severe wear and tear of the epidermis took place. The inner
opercular lining however did not show such massive necrotic
changes. Hyperplasia of the epithelial cells and great
vacuolization at various stages of exposure were the main
histopathological alterations.
Histopathological analysis of the sublethal toxicity induced
by 200 mg/L (10% of the 96-hour LC50 value) to the outer and
inner opercular epidermis was performed.
TEST ORGANISMS: Healthy individuals of H. fossilis (length
16-18 cm, body weight 35-40 g) collected from a single
population at Varanasi were acclimated in large plastic
aquaria for 3 weeks. Fish were fed with minced goat liver on
every alternate day. Water was renewed after every 24 hours,
leaving no fecal matter and unconsumed food. For
histopathological analysis, five groups of ten fish each
were exposed separately to 50 L of 200 mg/L [10% of the
96-hour LC50 value determined by trimmed Spearman-Karber
(with 5% trimming) method and 24 hours renewal bioassay
sytems] ammonium sulfate solution prepared in tap water
having pH 7.5, dissolved oxygen 6 mg/L, water hardness 23.2
mg/L and water temperature 22 +/- 22 °C. In the appropriate
control groups, no ammonium sulfate was added. Experimental
and control media were renewed after every 24 hours. Feeding
was allowed for control and experimental groups for 3 hours
before the renewal of the media. Five experimental and five
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