AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
not common test system
22-JUL-2004 (123)
Type:
other: effect on pulmonary function
Species:
sheep
Sex:
no data
Doses: 0.1 and 4.0 mg/m3, both concentrations tested at MMAD of 0.5
and 1.5 um
Exposure time: 4 hour(s)
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, not further specified
Result:
Pulmonary flow resistance was not affected by any aerosol
tested.
At 4.0 mg/m3, the airway responsiveness was enhanced only when
comparing challenge immediately or 24 h after ammonium sulfate
exposure to baseline values and when tested at a MMAD of 1.5
um, but not at 0.5 um.
Test condition: The effects of ammonium sulfate aerosols on pulmonary
resistance flow and airway responsiveness to inhaled carbachol
were studied in conscious sheep.
TEST ANIMALS: 28 adult ewes with a mean weight of 34 kg
(range 25-46 kg), 6-8 animals per test group.
MEASUREMENT OF RESPIRATORY MECHANISMS:
The unsedated, restrained sheep were intubated and a
catheter was placed into the lower esophagus for the
estimation of pleural pressure. Lateral pressure in the
trachea was measured with a sidehole catheter advanced
through and positioned distal to the tip of the endotracheal
tube. Mean pulmonary flow resistance was measured by
connecting the nasotracheal tube to a pneumotachograph.
AIRWAY RESPONSIVENESS:
Baseline pulmonary flow resistance was obtained after
inhalation of phosphate buffer. Bronchial challenges were then
performed using aerosolized solutions of phosphate buffered
carbachol. The airway responsiveness was defined as the
percentage increase from the baseline. Half an hour after
determination of airway responsiveness, animals were exposed 4
h to ammonium sulfate aerosol and immediately after exposure
and 24 h later baseline pulmonary resistance and airway
responsiveness were remeasured.
AEROSOL GENERATION:
A commercially available ultrasonic nebulizer was modified
for long term stability and used for the generation of two
particle sizes, MMAD 0.5 um and 1.5 um. Each particle size
was delivered at concentrations of 4 and 0.1 mg/m3. Humidity
was kept at 40%.
EXPOSURE TO TEST SUBSTANCE:
The sheep breathed the sulfate aerosols for 4 hours (head only
exposure). Some sheep were exposed to more than one salt and
in these instances the exposure were separated by at least one
week.
Reliability: (3) invalid
limited documentation, high interanimal variability, the
animals showed no reaction to H2SO4
10-APR-2006 (124)
156
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