AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
TEST METHODOLOGY: Twenty animals were kept per vessel, and
four replicates of each test were conducted, along with
controls. Bioassays were conducted after the method of APHA,
AWWA, and WPCF (1971): Standard method for the examination
of water and wastewater, 13th Ed. APHA Inc, New York, 874.
96 hour tests were carried out for insects and molluscs.
Test temperatures were 22.0 + 1.1 0C and 32.5 + 1.3 0C. 2000
organisms were used in this test, a similar test using
another substance, and the controls. The LC50 and LC 95
values were determined by probit analysis.
Reliability: (4) not assignable
Documentation insufficient for assessment. pH not measured.
Ammonium sulfate fertiliser characterisation is very
limited. Concentrations not measured. Number of
concentrations used and nominal concentration values not
stated. Limited documentation of results.
22-MAY-2003 (78)
4.3 Toxicity to Aquatic Plants e.g. Algae
Species:
Chlorella vulgaris (Algae)
Endpoint:
other: cell number
Exposure period: 18 day(s)
Unit: mg/l Analytical monitoring: no data
EC50: = 2700
Method:
GLP:
Test substance:
other: see Test Condition
no
other TS: ammonium sulfate, not further specified
Remark: In the control a lag-phase could be observed until day 11
(decline of cell number to approx. 10x10e6 cells/mL).
Logarithmic growth could be seen from day 11 - 18 (decline of
cell number up to appr. 70x10e6 cells/mL). From day 18 - 21
the cell number dropped back to 10x10e6 cells/mL. For this
reason the calculation of EC50 is referred to the cell number
at day 18.
Growth occurred in all ammonia concentrations (10-1000 mg
N/L), although less growth was found in cultures containing
either very low (10 mg N/L) or very high (750 and 1000 mg
N/L) ammonia concentrations.
Algal growth was accompanied by a decrease in nitrogen content
in the medium, indicating that nitrogen removal was due to
algal uptake and assimilation. Results demonstrate that C.
vulgaris can tolerate high concentrations of ammonia.
Result:
In the first about 10 days after start, in all test
concentration and in the control a lag phase was observed.
Thereafter in the control and some test concentrations an
exponential increase in cell number was observed up to day 17
or 18.
Based on data presented in graphics an EC50 (18 d, cell count)
of about 2700 mg/l could be calculated with "ToxRat Standard
Version 2.09 (Demo)". An EC10 could not be calculated by the
statistical program.
Test condition:
118
There are no data available to calculate the 72-hEC50.
STOCK CULTURE: Stock culture of Chlorella vulgaris was kept
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