AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
aseptically in a commercial Bristol medium (Bold Basal
medium,) containing 40 mg N /L as KNO3 and 53 mg P /L as
KH2PO4.
TEST MEDIA: The test media were prepared in the same way as
the Bristol medium except the nitrate component was replaced
by different amounts of ammonium sulfate.
A total of 12 ammonium concentrations were prepared: 0, 10,
20, 40, 50, 60, 80, 125, 250, 500, 750, and 1000 mg N/L. The
initial cell density was 1x10e6 cells/mL. The pH values of the
culture media were adjusted to 7.0 before algal
inoculation. The algae were grown in light-dark cycles of
16-8 hours for 21 days. The algal cell number was determined
at 3 or 4 day intervals.
The pH value was adjusted to 7 +/- 0.2 before algae
inoculation and was maintained at neutral pH during the study.
Reliability: (2) valid with restrictions
Flag:
Critical study for SIDS endpoint
23-AUG-2005 (80)
Species:
other aquatic plant: marine phytoplankton
Endpoint:
growth rate
Exposure period: 17 day(s)
Unit: mg/l Analytical monitoring:
GLP:
Test substance:
no
other TS: ammonium sulfate, not further specified
Remark:
The results demonstrate that marine phytoplankton is more
sensitive to ammonium sulfate than freshwater species, with
the marine dinoflagellates Gymnodinium splendens and Gonyaulax
polyedra showing growth inhibition in a 17d-test at
concentrations of 0.706 mg/L ammonium sulfate (calculated from
150 µg/L NH4-N) in a 17d-test. A reduction in photosyntheseis
within 3 hours is also shown for Gymnodinium splendens at
0.471 mg/L ammonium sulfate (calculated from 100 µg /L NH4-N).
No significance-levels are reported. A LOEC of 0.706 mg/L and
a NOEC of 0.471 mg/L (calculated from 150 and 100 µg/L NH4-N)
can be estimated for the endpoint growth reduction for
Gymnodinium splendens and Gonyaulax polyedra. Also, a LOEC of
0.471 mg/L and a NOEC of 0.235 mg/L (calculated from 100 and
50 µg/L NH4-N) can be estimated for the endpoint reduction in
photosynthesis for Gymnodinium splendens. No information on pH
in the test media is given.
Result: Species Exposure Time Effect
[mg (NH4)2SO4 / L]
Asterionella japonica up to 17 days NOEC > 0.942
Chaetoceros affinis up to 17 days NOEC > 0.942
Gymnodinium splendens up to 17 days NOEC = 0.471
LOEC = 0.706
Gonyaulax polyedra up to 17 days NOEC = 0.471
LOEC = 0.706
UNEP PUBLICATIONS 119