AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
pores, the number of nonciliated bronchiolar epithelial cells
per standardized area was counted, and means and SD were
calculated.
A section of the right cardiac lobe was prepared for
examination by light microscopy to identify any pathologic
processes.
Statistical analysis: two-way analysis of variance.
Reliability: (4) not assignable
no further information on purity, only one dose tested.
11-APR-2006 (128) (129)
Type:
Sub-chronic
Species: rat Sex: male
Strain:
Sprague-Dawley
Route of administration: inhalation
Exposure period:
4, 8 months
Frequency of treatment: 5 hours/d, 5 d/w
Post exposure period: half of the rats exposed for 8 months were maintained
for an additional 3-month recovery period
Doses:
0.5 mg/m3 (MMAD 0.44 um)
Control Group:
yes, concurrent no treatment
Method:
GLP:
Test substance:
Remark:
Result:
other: see Test Condition
no data
other TS: ammonium sulfate, no further details given
The study was designed to investigate effects of ammonium
sulfate in normal animals and animals pretreated with
elastase, which results in lung emphysema. Only the results
for ammonium sulfate vs. air controls are reported in detail.
No significant differences were found in final body weights
or some immunologic parameters measured after 4 months only
(spleen weight, mitogenic response of spleen cells or
peripheral blood lymphocytes, distribution of T cells in the
spleen). No differences were found in vital capacity, total
lung capacity, time constant, CO2 diffusion capacity, residual
volume, functional residual capacity or N2-slope. The
quasistatic compliance was reduced significantly. Only after 4
months was the incidence and severity of bronchiolar
epithelial hyperplasia significantly increased. Non ciliated
epithelial cell counts were increased significantly after 4
and 8 months. Chord length means and medians were increased
after 4 months significantly but only numerically after 8
months. However, after the recovery period, these differences
were significant. After 8 months, emphysema incidence and
severity were increased only numerically whereas alveolar
interstitial birefringence, a measure of fibrosis, was
increased significantly. Elastase pretreatment seems to have
repressed or obscured the effects of ammonium sulfate.
Morphological lung effects in saline-pretreated rats after
exposure to ammonium sulfate (AS) for 4 months, 8 months, or 8
months plus 3 months recovery:
___________________________________________________________
Exposure / 4 months / 8 months / 8 + 3 months /
-----------------------------------------------------------
N / Air AS / Air AS / Air AS /
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166
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