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AMMONIUM SULFATE CAS N°: 7783-20-2

AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

tissue culture plate which was used with a disecting<br />

microscope. A stock solution of ammonium sulfate was freshly<br />

prepared, and used for further dilutions. 4 to 6 miracidia<br />

were picked up in 1 ml dechlorinated water, and placed in<br />

each well of the test chamber. 1 ml of double strength<br />

ammonium sulfate solution was then added,according to the<br />

test schedule, giving a total of 2 ml in each experimental<br />

well. 6 Replicate concentrations were employed. The test<br />

concentrations were 0 (control), 0.1, 0.2, 0.3, 0.4, and<br />

0.5% ammonium sulfate. Careful hourly observations were made<br />

under the disecting microscope. Motionless miracidia (round<br />

shaped in the control group and elongated in the<br />

experimental group) were recognised as dead.<br />

STATISTICAL ANALYSIS: The EPA Probit statistical package<br />

(EPA Probit Analysis Program Version 1.3) as used to<br />

calculate LC5, LC50, and LC95 values after 2, 4, and 6 hours<br />

of exposure.<br />

Reliability: (2) valid with restrictions<br />

Only the LC50 result at 4 hours is statistically robust.<br />

Concentrations not measured. Limited documentation of<br />

results.<br />

22-MAY-<strong>20</strong>03 (77)<br />

Species:<br />

other aquatic worm: planaria tigrina<br />

Exposure period: 1<strong>20</strong> hour(s)<br />

Unit: mg/l Analytical monitoring: no data<br />

EC50: = 97<br />

EC100: = 150<br />

EC5 : = 41<br />

Method:<br />

GLP:<br />

Test substance:<br />

other: see Test Condition<br />

no data<br />

other TS: ammonium sulfate, analytical grade<br />

Test condition: Acute toxicity tests were performed with various materials<br />

used as fertilizer in Czechoslovakia.<br />

The tests were performed at pH 7.5 to 7.8 at 19-22 degree C<br />

with Planaria tigrina Girard. No detail is provided as to<br />

the experimental procedure (no. of test organisms, exposure<br />

etc.).<br />

Reliability: (4) not assignable<br />

Abstract, original reference in foreign language<br />

26-JAN-<strong>20</strong>04 (68)<br />

Type:<br />

static<br />

Species:<br />

other: Belostoma sp. (aquatic insect)<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: no<br />

Limit Test: no<br />

Method:<br />

other: see Test Condition<br />

GLP:<br />

no data<br />

Test substance: other TS: commercial grade ammonium sulphate containing <strong>20</strong>.6%<br />

nitrogen.<br />

Remark: Inconsistent information in test methodology described -<br />

between the number of animals per vessel and the total<br />

number of animals used in the test - for a valid testing<br />

UNEP PUBLICATIONS 109

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