AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
at the end of the test. The means and standard deviations
of the ammonium-nitrogen concentrations measured at the 5
experimental concentrations and in the control were 211.5 +
16.3, 126.5 + 4.9, 81.5 + 7.8, 52.5 + 4.9, 32.4+ 3.3 and 0
mg/l ammonium nitrogen, respectively. Ten day LOEC values
and NOEC values were determined with the Dunnetts multiple
comparison procedure.
Reliability: (1) valid without restriction
Flag:
Critical study for SIDS endpoint
22-JUL-2004 (67)
Species:
Expos. period:
Method:
GLP:
Test substance:
Result:
Test condition:
other: Ambystoma gracile (Northwestern salamander)
10 day(s)
other: see Test Condition
no data
other TS: ammonium sulfate, 99.2% pure (Reagent Grade,
Mallinckrodt Baker Inc, Phillipsburg, NJ)
The NOEC for the larvae, which were 5 weeks old at the
start of the 10 day test, was 81.5 mg/L ammonium nitrogen.
The corresponding LOEC was 126.5 mg/L ammonium nitrogen.
TEST ORGANISMS: Eggs of Ambystoma gracile were collected
locally from non-agricultural areas and hatched in the EPA
laboratory facilities at Corvallis, Ore. They were
subsequently reared in aquaria with a continuous flow of
temperature controlled fresh water whose temperature was
gradually increased from the collection temperature of ca.
10 deg C to the test temperature of 20 +/- 1 deg C.
Temperature was continuously recorded. They were held at a
photoperiod of 14:10 light:dark. Newly hatched Ambystoma
gracile were fed newly hatched brine shrimp, and then
daphnids (D. pulex and D. magna) and annelid worms
(Lumbriculus variegatus) as they grew larger.
TEST VESSEL: The test species were exposed to ammonium
sulfate in 18 10-L aquaria with a continuously-flowing water
diluter system that automatically delivered six
concentrations of chemical and controls to three aquaria per
concentration.
PHYSICAL/CHEMICAL PARAMETERS: Rearing and test water was
obtained from wells near the Willamette River at Corvallis,
Oregon. Dissolved oxygen, measured by electrode, was
maintained near saturation by the flowing water. pH (median
value 7.2) was measured by electrode. Total hardness (34 +/-
0 mg/L), alkalinity (34 +/- 0 mg/L), and conductivity (104.4
+/- 1.2 µS/cm) were measured by EPA methods 130.2, 310.1,
and 120.1, respectively. Background ammonium-N ranged from
0.005 - 0.010 mg/L in well water, and from 0.00 to 0.03 mg/L
in control tanks. Water samples for measurement of ammonium
sulfate were taken from each concentration (each aquarium in
the diluter) on two days, and analysed with a Hach DR/700
digital photometer. Quality assurance techniques were used
in the analysis.
TEST CONDITION: Four 5-week old larvae were used in each of
three replicate aquaria, and the test was run for 10 days.
Length and mean wet wet were measured at the end of the
test. The means and standard deviations of the
ammonium-nitrogen concentrations measured at the 5
experimental concentrations and in the control were 211.5
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