AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
control fish each were sacrificed after 5, 10, 20, 30, and
45 days of exposure. Opercula were fixed in 10% neutral
formalin, Bouin`s fluid and Helly`s fluid. Six µm paraffin
sections were stained with Ehrlich`s hematoxylin/eosin for
routine histopathological analysis, periodic acid Schiff
(PAS) for neutral glycoproteins, alcian blue pH 2.0 for
sulfated mucopolysachharides, alcian blue pH 2.5 for acidic
glycoproteins, alcian blue 2.5/PAS dual staining for neutral
and acidic glycoproteins and bismarck brown for
water-resistant mucoproteins. Morphometric measurements were
taken using an oculometer and stage micrometer. Standard
statistical procedures based on random sampling from 10
different sections from all the five fish of each stage of
all the experimental and control groups were performed. One
way analysis of variance (ANOVA) followed by Duncan`s
multiple range test were done for multiple comparison. Since
the differences between the measurements taken from various
control groups at different time intervals of the exposure
were not significant, the average of all the control groups
was taken into consideration.
Reliability: (2) valid with restrictions
limited documentation
02-FEB-2004 (64)
Type:
static
Species:
Cyprinus carpio (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
Method:
GLP:
Test substance:
Result:
Test condition:
90
other: see Test Condition
no data
other TS: ammonium sulfate, commercial grade
96-hour LC50 values for various life stages of C. carpio at
different temperatures:
20 °C: egg 70 mg/L, spawn 101 mg/L, fry 120 mg/L.
24 °C: egg 67 mg/L, spawn 124 mg/L, fry 140 mg/L.
28 °C: egg 60 mg/L, spawn 78 mg/L, fry 93 mg/L.
32 °C: egg 18 mg/L, spawn 52 mg/L, fry 121 mg/L.
36 °C: egg 23 mg/L, spawn 48 mg/L, fry 45 mg/L.
After six days following eyposure, all embryos died within the
sac. No abnormal development exhibited to other temperatures.
Organ formation was not observed.
The results indicated that eggs were more sensitive to
ammonium sulfate than spawn and fry at all temperatures. The
authors surmised that the inhibitory effects of ammonium
sulfate on the development of eggs at high temperatures may be
due to the interaction of several metabolites like ammonia
nitrate and nitrite with various enzymes in the embryo.
Spawn and fry (0.8 +/- 0.02 g) of C. carpio were acclimated
to laboratory conditions for 7 days. Eggs were collected
from an unfertilized pond. Five temperatures within the
natural temperature range of streams and ponds in India were
used in the study.
The experiments were performed for 96 hours in 500 mL glass
beakers according to the APHA (1975) methods, using
unchlorinated tubewell water (DO 7.5 ppm, total alkalinity
200 ppm as CaCO3, pH 7.5).
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