AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Conclusion:
preparations was determined by the selective incorporation
of tritium-uridine. Four T- and B-cell specific mitogens
were utilized for the mitogen-induced activation test
(concanavalin A (Con A), phytohemagglutinin (PHA), pokeweed
mitogen (PWM), lipopolysaccharide (LPS)).
STATISTICAL ANALYSES. two-way analysis of variance, analysis
of covariance, Fisher´s exact test. Immunologic studies did
not involve comparisons of lung condition, and only the
differences among the four exposure groups were tested by
analysis of variance.
A series of studies in which rats and guinea pigs were exposed
to 1 or 0.5 mg/m3 for up to 8 month describes
histomorphometrically determined increases in emphysema
formation, hyperplasia of non-ciliated cells in the
bronchiolar epithelium and increased interstitial
birefringence indicating increased collagen content. The
findings showed a high variation within the control animals
and no consistent time-response pattern. There was no close
correlation between the morphological findings and lung
function tests, most of which did not show differences between
control and treated groups. Therefore these findings cannot
definitively be attributed to the test substance treatment.
This conclusion is supported by the fact that ammonium sulfate
is a highly soluble material with a moderately acidic pH
(around 5), which is rapidly neutralized and absorbed when
deposited in the respiratory tract. It is thus not expected to
show cytotoxicity.
Reliability: (4) not assignable
no information on purity, only one dose tested.
11-APR-2006 (128) (130)
Type:
Sub-acute
Species: rat Sex: male
Strain:
Sprague-Dawley
Route of administration: inhalation
Exposure period:
23.5 h up to 7 days
Doses:
5 mg/m3 as aerosol
Result:
The apparent lung collagen synthesis rate, assayed by the
incorporation of tritium labeled proline into hydroxyproline
of lung collagen, was increased to 35 nmol/g at 0.64 ppm
ozone, which was about 200% above the control values. Total
protein content of bronchoalveolar lavage reached a maximum of
about 10 mg, a 590% increase over the control levels after a 1
day (23.5h) exposure to 0.64 ppm of ozone. The level declined
to near base amounts after 7 days. Lower ozone doses elicited
proportionally smaller increases in the total protein. The
lung protein content increased to about 140 mg at the high
ozone level after 7 days. The lung DNA content was elevated to
125% over the base amount at 0.64 ppm, the 0.12 and 0.20 ppm
doses showed no increase. The exposure to 0.20 ppm of ozone
and about 5 mg/m3 of ammonium sulfate resulted in a 125%
increase in collagen synthesis. Exposures to 5 mg/m3 of
Ammonium sulfate alone have been shown previously not to cause
changes in collagen synthesis rate as compared with control.
Seven day exposure to the high level of ozone increased the
UNEP PUBLICATIONS 169