AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
segregated in welded stainless steel, 55-mesh wirecloth
baskets (5.5 cm in diameter x 7.5 cm in depth). Each basket
was suspended from a 1-rpm motor-driven mechanism that
raised and lowered the baskets in the water column. A
stainless steel band, slotted every 0.5 cm, facilitated the
positioning of the baskets so that the submerged volumes
changed from one-third to wo-thirds during each cycle.
One-half of the volume of the sumerged basket was exchanged
with the main tank volume every minute.
TEST SOLUTION: The test chemical was added directly to the
diluent water to give a nominal concentration of 100 mg/L.
The test chemical concentrations were not analyzed. Once
thetest solutions were prepared, the starting temperature,
dissolved oxygen and pH values were determined for each
exposure concentration. When the starting pH of the test
soluton fell outside the extremes of 6.5 to 8.5, the pH was
adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%
(v/v) H2SO4.
DILUTION WATER: Water quality was routinely monitored to
characterize the diluent water and ensure its suitability
according to the standard method for the examination of
water and wastewater, 16th ed., American Public Health
Association, Washington DC pp. 689-832. The chemical
characteristics are reported in the publication and are in
accordance with standards required by current testing
guidelines. Activated carbon-filtered, dechlorinated and
tempered industrial service water from Lake Ontario was used
in all tests.
PHYSICAL/CHEMICAL PARAMETERS:
Determination of the temperature, dissolved oxygen and pH of
each test solution were made in conjunction with the daily
biological observation. The test temperature target was 20 +/-
1 °C. If the dissolved oxygen concentration in a test chamber
fell below 40% of the starting level in a test, the test was
repeated with 0.05 L/min glass-sparger aeration All tests were
conducted within the extremes of 6.5 to 8.5 pH units. The
photoperiod duration was 16 h of light. The air-water
interface of each tank received approximately 50 ft-c of
cool-white fluorescent light.
BIOLOGICAL PARAMETERS: biological observations were made
daily. Survival, condition and behavioural information were
recorded. Dead organism were removed when observed. If more
than one-half of the population exposed in any treatment was
found to be dead, additional aquaria containing lower
concentrations of test soution were set up.
EXPOSURE PERIOD: 96 hours.
TEST CONCENTRATION: 100, 10, 1 and 0.1 mg/l
STATISTICAL METHOD: The 96-h LC50 value was derived using an
interpolation method as described by Stephan (Stephan CE:
Methods for calculating an LC50: Aquatic toxicology and
hazard evaluation. In: FL Mayer and JL Hamelink, eds., STP
634. American Society for Testing and Materials,
Philadelphia, PA, pp. 65-84). The linear interpolation uses
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