AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Result:
96-h LC50 (September 1977): 210 mg/L,
96-h LC50 (December 1977): 200 mg/L
96-h LC50 (June 1978):130 mg/L
Test condition: The fish were caught in the estuary of the River Crouch by
the Laboratory`s research vessel using a modified 2 m beam
trawl with 10 mm mesh in the cod end. On arrival in the
laboratory the animals were tranferred to polyethylene stock
tanks at a maximum density of 100 fish per tank.
Water at the test emperature of 15 °C was used to slowly
fill the tanks and any dead or injured animals were removed.
The animals were maintained in well aerated, gently flowing
sea water for 2-4 days before the start of the test. They
were not fed during their period in the laboratory, which
never exceeded 9 days.
Test tanks were filled with 10 L of sea water and aerated
for at least 1 hour. Ten fish of 50-100 mm total length
(about 2-8 g) were then randomly added to each tank.
Diseased animals were excluded. A further acclimatisation
period of 2 hour was allowed before the test solutions were
added.
Test solutionswere made up by gently stirring a measured
amount of the well mixed test substance into the sea water
in the test tanks.
Ammonium sulfate was added to the test tanks as 10% w/v
solution in distilled water. Four concentrations were set up
initially, with controls of clean sea water. Duplicates were
used at each concentration.
The concentrations chosen started at 10,000 uL/L (1%) and
decreased at half-logarithmic intervals (ie 3300, 1000 and
330 uL/L).
Test solutions were renewed daily to discard metabolites and
counteract losses of the test substance due to absorption by
the test organisms, degradation or volatilization.
The dissolved oxygen concentration in each test tank was
measured and recorded daily. If the dissolved oxygen
concentration in any of the test tanks dropped below 70% air
saturation value then the test was repeated with additional
aeration.
The pH of the test solution was measured and recorded 1 and
24 hours after the start of the test, but is not reported in
the paper. The intended pH range was between pH 6 and pH 8.
Each test continued for 96 hours.
The tanks were inspected at frequent intervals, including
24, 48, 72 and 96 hours after adding the test solution, and
dead animals (defined as those not responding to gentle
prodding) were recorded and removed. At the time of each
observation and for each tank the cumulative percentage
mortality was calculated. The time for 50% lethality (LT50)
and the LC50s were determined. 95% confidence limits on the
LT50 were calculated according to the method of Litchfield
(1949).
The three tests with ammonium sulfate were carried out in
September and December 1977, June 1978.
Reliability: (2) valid with restrictions
limited documentation, nominal concentrations; pH not given.
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (60)
Type:
static
86
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