AMMONIUM SULFATE CAS NÂ°: 7783-20-2

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OECD SIDS AMMONIUM SULFATE 4. ECOTOXICITY ID: 7783-20-2 DATE: 18.04.2006 Test condition: killed at the highest dose level (100 mg/L) was less than 50%. No further details reported. TEST ORGANISMS: Snail (Helisoma trivolvis), 0.180 g. The test organisms were acclimated to the control diluent water in the breeding/rearing tanks (time period not reported). Food was withheld for the 24 hours preceding start of the test. Juveniles as uniform in size as possible were collected from the colonies. Ten juvenile organisms were exposed to the test chemical in each treatment. Biological loading was kept below 0.5 gram wet weight per liter of test solution. TEST VESSEL: The assay was performed in seamless glass, 30.5-cm cuboidal, Pyrex chromatographic jars to which 20 liters of test solution was added. The test organisms were placed directly in the aquaria. TEST SOLUTION: The test chemical was added directly to the diluent water to give a nominal concentration of 100 mg/L. The test chemical concentrations were not analyzed. Once the test solutions were prepared, the starting temperature, dissolved oxygen and pH values were determined for each exposure concentration. When the starting pH of the test soluton fell outside the extremes of 6.5 to 8.5, the pH was adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10% (v/v) H2SO4. DILUTION WATER: Water quality was routinely monitored to characterize the diluent water and ensure its suitability according to the standard method for the examination of water and wastewater, 16th ed., American Public Health Association, Washington DC pp. 689-832. The chemical characteristics are reported in the publication and are in accordance with standards required by current testing guidelines. Activated carbon-filtered, dechlorinated and tempered industrial service water from Lake Ontario was used in all tests. PHYSICAL/CHEMICAL PARAMETERS: Determination of the temperature, dissolved oxygen and pH of each test solution were made in conjunction with the daily biological observation. The test temperature target was 20 +/- 1 °C. If the dissolved oxygen concentration in a test chamber fell below 40% of the starting level in a test, the test was repeated with 0.05 L/min glass-sparger aeration All tests were conducted within the extremes of 6.5 to 8.5 pH units. The photoperiod duration was 16 h of light. The air-water interface of each tank received approximately 50 ft-c of cool-white fluorescent light. BIOLOGICAL PARAMETERS: biological observations were made daily. Survival, condition and behavioural information were recorded. Dead organism were removed when observed. If more than one-half of the population exposed in any treatment was found to be dead, additional aquaria containing lower concentrations of test soution were set up. EXPOSURE PERIOD: 96 hours. TEST CONCENTRATION: 100, 10, 1 and 0.1 mg/l 106 UNEP PUBLICATIONS
OECD SIDS AMMONIUM SULFATE 4. ECOTOXICITY ID: 7783-20-2 DATE: 18.04.2006 STATISTICAL METHOD: The 96-h LC50 value was derived using aninterpolation method as described by Stephan (Stephan CE: Methods for calculating an LC50: Aquatic toxicology and hazard evaluation. In: FL Mayer and JL Hamelink, eds., STP 634. American Society for Testing and Materials, Philadelphia, PA, pp. 65-84). The linear interpolation uses the logarithm transformation of the concentration and the angle transformation of the percent dead between the two doses that bracket 50% response. YEAR OF STUDY: not reported. Reliability: (2) valid with restrictions concentration not measured. Limited documentation of results. 26-JAN-2004 (59) Type: static Species: other aquatic mollusc: Lamellidens marginalis Exposure period: 48 hour(s) Unit: mg/l Analytical monitoring: no data LC50 : = 3528 Method: other: static test as described in Doudoroff (1951), Sewage Wastes 23, 130 Year: 1995 Test substance: other TS: ammonium sulfate, purity 99% Test condition: TEST ORGANISMS: Freshwater mussels, belonging to the species Lamellidens marginalis weighing 30 +/- 2 g, collected from the ponds of an Indian village. The animals were fed ad libitum on freshwater plankton and acclimated to laboratory conditions for a period of 15 days. Concentration (sublethal): 10 and 176.4 mg/l about 7 days EXPOSURE TO TEST SUBSTANCE: Batches of 10 animals were exposed to different concentrations of ammonium sulfate ranging from 1,000 to 5,000 mg/L with an interval of 100 mg/L. Each experiment in the selected concentrations of ammonium sulfate was repeated six times. OBSERVATIONS: After 48 hours the number of animals died at each concentration was recorded. the animal was considered dead when the mantle edge was no longer responsive to touch and failure to close the shell valves. DETERMINATION OF THE LC50: The mortality in each concentration was taken to determine the LC50 by graphical plots of per cent mortality, probit mortality against log concentration of ammonium sulfate according to the method of Finney, 1964. Reliability: (2) valid with restrictions limited documentation 26-JAN-2004 (76) Type: static Species: other aquatic worm: Schistosoma mansoni Exposure period: 4 hour(s) Unit: Analytical monitoring: no UNEP PUBLICATIONS 107
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OECD SIDS AMMONIUM SULFATE FOREWORD
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OECD SIDS AMMONIUM SULFATE 10. Comm
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OECD SIDS AMMONIUM SULFATE Similarl
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OECD SIDS AMMONIUM SULFATE Consumer
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OECD SIDS AMMONIUM SULFATE 1.3 Phys
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OECD SIDS AMMONIUM SULFATE - in the
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OECD SIDS AMMONIUM SULFATE The inte
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OECD SIDS AMMONIUM SULFATE 228 µg/
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OECD SIDS AMMONIUM SULFATE also an
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OECD SIDS AMMONIUM SULFATE 3.1.3 Ir
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OECD SIDS AMMONIUM SULFATE A simila
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OECD SIDS AMMONIUM SULFATE diarrhoe
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OECD SIDS AMMONIUM SULFATE oral exp
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OECD SIDS AMMONIUM SULFATE Table 3
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OECD SIDS AMMONIUM SULFATE No test
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OECD SIDS AMMONIUM SULFATE freshwat
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OECD SIDS AMMONIUM SULFATE Toxicity
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OECD SIDS AMMONIUM SULFATE Table 7
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OECD SIDS AMMONIUM SULFATE 6 REFERE
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OECD SIDS AMMONIUM SULFATE Gmelin (
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OECD SIDS AMMONIUM SULFATE I U C L
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OECD SIDS AMMONIUM SULFATE 1. GENER
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AMMONIUM SULFATE CAS NÂ°: 7783-20-2

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