AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
slow, suppressive waves and high-amplitude slowing wave
patterns. There was a remarkable increase in the
concentration of ammonium ion and inorganic sulfate ion in
serum, and blood gas analysis showed severe metabolic
acidosis.
Electrolytes did not show any significant change except for
a moderate increase in K+ just before cardiac arrest.
Concentrations of AST, ALT and LDH remained constant and
normal levels were maintained throughout the experiment.
Also, the values of BUN and creatinine were always within
normal ranges.
No pathological changes were found in brain, heart, lung,
spleen, kidney, liver and stomach.
Test condition: Five Japanese white rabbits, weighing 3.4 to 3.8 kg were
used. The animals were anesthetized with intravenous
pentobarbital sodium (12.5 mg/kg bw), injected in auricular
veins, and supplemental doses were intermittently given to
maintain surgical level of anesthesia. Rabbits were fixed in
supine position and a double channel catheter was placed in
the femoral artery to monitor blood pressure and to draw blood
samples. Electrocardiogram and electroencephalogram were
recorded from needle electrodes. Respiration rate was counted
by movement of the thorax. The total dose of 1500 mg/kg
ammonium sulfate (dissolved in saline solution) was
administered in 10-15 mL of volume per rabbit through gastric
probe in three rabbits. 2 rabbits were administered 10-15 mL
of saline and used as controls.
Blood samples were collected from the femoral artery every
15 minutes up to 90 minutes after administration of ammonium
sulfate solution including 5 minutes before and 5 and 10
minutes after ingestion.
Blood gas was analyzed with heparinized blood and the serum
level of ammonium ion, AST, ALT, LDH, BUN and creatinine, as
well as electrolytes of Na, K, Cl and Ca were measured.
Inorganic sulfate ions in serum were measured by ion
chromatography.
Brain, heart, lung, spleen, kidney, liver and stomach were
removed after death, and microscopical sections were
prepared for histopathological examination with HE- and
Elastica Masson stains.
Year of study: not reported.
Reliability: (2) valid with restrictions
limited documentation, only one dose tested
11-APR-2006 (105)
5.1.2 Acute Inhalation Toxicity
Type:
other: IRT (inhalatory risk test)
Species:
rat
Strain:
no data
Sex:
no data
No. of Animals: 12
Exposure time: 8 hour(s)
Method:
other: see Test Condition
Year: 1969
GLP:
no
Test substance: other TS: as prescribed by 1.1 - 1.4, "chemically pure"
150
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