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AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

fingerlings of the same species.<br />

Results from the tests with hatchlings were as follows:<br />

- C. carpio: LC50 77 mg/L (LC5 13 mg/L, LC95 141 mg/L;<br />

n=160).<br />

- L. rohita, C. catla, C. mringala: LC50 49 mg/L (LC5 7<br />

mg/L, LC95 85 mg/L; n=146).<br />

Test condition: Fishes were purchased from local farms and were acclimated<br />

to the laboratory conditions for 96 hours. The mean lengths<br />

and weights of the fishes used were as follows:<br />

- Labeo rohita 48+/- 3.2 mm, 1.634 +/- 0.183 g<br />

- Catla catla 49 +/- 1.5 mm, 3.495 +/- 0.311 g<br />

- Cirrhinus mrigala 24 +/- 1.1 mm, 0.46 +/- 0.046 g<br />

- Cyprinus carpio 41 +/- 1.5 mm, 1.374 +/- 0.076 g<br />

- Tilapia mossambica 42 +/- 2.5 mm, 1.719 +/- 0.197 g.<br />

Hatchlings of major carps and Cyprinus carpio were<br />

acclimated for 2 hours to the laboratory conditions.<br />

The experiments were performed in the laboratory at 30.8 +/-<br />

1.2 °C, using unchlorinated borehole water (DO 9.9, total<br />

alkalinity 290 ppm as CaCO3, pH 7.5).<br />

Tests were run for 96 hours in 15 L glass aquaria for<br />

fingerlings and in 500 mL glass beakers for hatchlings<br />

according to the APHA methods (1975). Two to four fishes in<br />

each aquarium and 8-16 fishes per concentration, and 25<br />

hatchlings in each beaker were tested.<br />

All tests were repeated five to eight times acompanied with<br />

controls.<br />

The following chemicals were tested: urea, ammonium sulfate,<br />

superphosphate, muriate of potash, and lime.<br />

Reliability: (2) valid with restrictions<br />

limited documentation<br />

23-AUG-<strong>20</strong>05 (62)<br />

Type:<br />

semistatic<br />

Species:<br />

other: Heteropneustes fossilis<br />

Exposure period: 10 day(s)<br />

Unit: mg/l Analytical monitoring: no data<br />

Method:<br />

GLP:<br />

Test substance:<br />

Result:<br />

Test condition:<br />

88<br />

other: see Test Condition<br />

no data<br />

other TS: ammonium sulfate, not further specified<br />

The goblet cells show cyclic increased (due to hyperplasia<br />

and hyperactivity) followed by decrease (due to exhaustion<br />

and degenration) mucogenic activity. Cyclic hemorrhage takes<br />

place due to rupture of the tips of the secondary lamellae,<br />

which also regenerate several times. This causes hyperplasia<br />

of the haphazardly arranged epithelial cells, leading to<br />

decreased secondary lamellar density. Uncomtrolled<br />

hyperplasia also causes increased distance of respiratory<br />

blood-air barrier, which along with decreased lamellar<br />

density results in impaired aerial respiration, leading to<br />

asphyxiation and ultimate death of the fish.<br />

H. fossilis (length 16 +/- 2 cm) collected locally were<br />

acclimated in the laboratory for <strong>20</strong> days in tap water in<br />

plastic aquaria. They were regularly fed with minced goat<br />

liver. Feeding was discontinued one day before the start of<br />

the experiment. Ten groups of 10 fish each, irrespective of<br />

their sex, were exposed to 50 L of <strong>20</strong>00 ppm of ammonium<br />

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