AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
(nose, larynx, bronchii, the lobes of the lung) were prepared,
and hematoxylin- and eosin- stained slides were evaluated.
The study was repeated and the results of both studies with
160 animals was reported in the EPA report (1978) and with 80
animals in the literature of Godleski. (1984).
Reliability: (4) not assignable
The interval between termination of exposure and examination
was too long, therefore the reliability is 4
Flag:
Critical study for SIDS endpoint
12-APR-2006 (104) (137)
Type:
Sub-acute
Species: guinea pig Sex: male
Strain:
Hartley
Route of administration: inhalation
Exposure period:
4 weeks
Frequency of treatment: 6 hours/day, 5 days/week
Post exposure period: no
Doses:
1.03 mg/m3; MMAD 0.42 um
Control Group:
other: sham exposure
Remark:
Result:
Test condition:
180
According to the study authors, the described alterations in
the secretory response may have protected the lungs of
exposed animals from the adverse effects of inhaled aerosols
and may have contributed to the functional differences
observed between ammonium sulfate exposed rats (see study
Busch et al. 1984) and guinea pigs.
An apparent alteration in secretory activity characterized
by hypertrophy and hyperplasia of nonciliated epithelial
cells, with an increased number of secretory granules per
cell was observed in the lungs of animals exposed to
ammonium sulfate aerosol. These changes were seen in
airways ranging from small bronchi to terminal respiratory
bronchioles.
Young adult animals (Charles River Lab., Kingston, NY) were
maintained in isolation for 3 weeks prior to beginning
experimental procedures. Animals were assigned to four
experimental groups based on their initial weights, so that
the distribution of animal weights for each treatment group
was the same. The animals were divided into two groups, one
to receive intratracheally instilled porcine pancreatic
elastase ("elastase impaired") and one to receive saline
solution intratracheally (controls). 30 guinea pigs received
5 units of elastase activity / 100 g bw. The dose level was
determined in preliminary experiments to produce a
predictable degree of emphysema without inducing significant
mortality. No data about number of saline treated guinea pigs
are evaluable.
Sterile elastase or saline solutions, standardized for
activity (in the case of elastase), osmolarity, pH, and
temperature, were administered intratracheally to
ether-anesthetized animals. The total volume of the
instillate varied slightly, depending on animal weight;
however, concentration/volume was constant. Following
recovery from anesthesia, the animals were caged for a
3-week recovery period.
EXPOSURE TO TEST SUBSTANCE:
One-half of the elastase-treated animals and one-half of the
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