AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Endpoint:
growth rate
Exposure period: 14 day(s)
Unit: mg/l Analytical monitoring: no data
LOEC: = 100
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, not further specified
Result:
Supplementation of ammonia (from ammonium sulfate) as
nitrogen source did not support growth at all. The growth in
ammonium sulfate containing medium (50 and 100 mg/L) had a
prolonged lag phase, which was followed by a very short
exponential and stationary phase, after which the cultures
started to turn chlorotic at 14 days of culture.
Test condition: TEST ORGANISMS: unicellular blue-green alga Synechococcus
cedrorum IU1191, obtained from the Indiane University
Culture collection.
To study the effect of inorganic nitrogen compounds on
growth of algae, sodium nitrate, sodium nitrite and ammonium
sulfate were used separately in the following
concentrations: sodium nitrate: 0.05 g/L and 1.5 g/L
(control); sodium nitrite: 0.5 g/L and 0.75 g/L; ammonium
sulfate: 0.05 g/L and 0.1 g/L. The cultures were grown under
aseptic conditions in Hughe`s medium with sodium nitrite,
sodium nitrate or ammonium sulfate as nitrogen source at pH
7.8 and 26 °C with 1400 lux light intensity. The cultures
were incubated in side arm flasks (a specially designed
conical flask with a side arm or tube), each containing 20
mL of growth medium. Growth was measured by measuring
cellular absorbance at 700 nm with a photoelectric
colorimeter.
Reliability: (3) invalid
invalid testdesign and insufficient documentation
13-JUL-2004 (83)
4.4 Toxicity to Microorganisms e.g. Bacteria
Type:
Species:
Unit:
Method:
GLP:
Test substance:
Remark:
Result:
Test condition:
field
other bacteria: nitrogen-fixing cyanobacteria
Analytical monitoring: yes
other: see Test Conditions
no data
other TS: ammonium sulfate as fertilizer, no further details
The result will be influenced by the pH (4.4) of the soil,
which results from ammonium sulfate addition. Increased
ammonia volatilisation at acid pH may contribute to the
effect.
Applied ammonium sulfate at 17.5, 35, 70, and 140 kg N /ha
reduced the numbers of cyanobacteria and the nitrogen
fixation observed. However, the ammonium sulfate inputs of
up to 70 kg N /ha gave rise to a moderate increase in grain
yield, without severely affecting the N2 fixing ability
(less than 50% reduction) or cyanobacteria numbers.
EXPOSURE PERIOD:
mean of 3 yearly experiments.
UNEP PUBLICATIONS 121