AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
- 20 µg/m3: slightly, but not significantly, elevated albumin
level in the bronchoalveolar lavage fluid as indication for an
increased permeability of the epithelium
- 70 µg/m3: no effect
HISTOPATHOLOGICAL INCIDENCE AND SEVERITY:
Lung morphometric analysis:
20 µg/m3: not studied
70 µg/m3:
- Alveolar wall nuclear density was increased slightly, but
not significantly
- Alveolar septal wall thickness was increased significantly
(ca: 20%, only graphic results given)
- Both alveolar chord length (a measure of average alveolar
linear. dimension), and alveolar cross-sectional area tended
to decrease.
- Significant decrease in alveolar cross-sectional area (ca.
7%, only graphic results given)
The number of goblet cells in the respiratory epithelium was
unaffected by the treatment.
No histopathology (light microscopic investigations) was
performed or described, respectively, so the cause of the
increase in thickness of the alveolar septal wall remains
unclear. These results do not account for any kind of
emphysema.
Test condition:
No final assessment is possible because of incomplete
documentation.
The study analyzed the effects of three PM-10 components
(ammonium sulfate, ammonium nitrate, and road dust).
ANIMALS: F344/N rats (Simonsen Laboratories, Inc., Gilroy,
CA) [note: in the study abstract the use of Sprague-Dawley
rats is stated].
Age and Weight at study initiation: not reported.
Number of animals per sex per dose: not reported; 12
animals/group were used for the macrophage function analysis;
no information is available on the number of animals and the
sex of animals used in the other experiments. For all
experiments (i.e. including those performed with road dust and
ammonium nitrate), a total of 144 rats were used.
TEST PROCEDURE:
Ammonium sulfate aerosols were generated by nebulization of
dilute aqueous solutions. The particles were mixed with dry
dilution air, and equilibrated to 60% relative humidity.
Rats were exposed nose-only. The high-dose group was tested in
one set of exposures and the low-dose group was tested in a
separate set of exposures. Separate purified air control
groups were used for high-and low-concentration exposures.
Rats were killed 1-2 h after the end of exposure.
CLINICAL OBSERVATIONS: not reported
EXAMINED PARAMETERS:
Separate groups were assessed for histopathology endpoints and
for macrophage and permeability-related end-points.
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