AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
abaxial and adaxial leaf resistances were decreased from
control values.
Test condition: TEST ORGANISMS: Pinto bean plants (Phaseolus vulgaris L.)
14 days in age at the beginning of the test. All plants were
staked to ensure an erect growth habit.
TEST VESSEL: environmental growth chamber, Daytime climate
45-55% r.h. 250C. Night-time climate 70-80% r.h. 200C.
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (26)
Species:
Expos. period:
Unit:
Method:
GLP:
Test substance:
other terrestrial plant: Orobanche crenata Forsk
7 day(s)
mmol/l
other: laboratory test, see Test Conditions
no data
other TS: ammonium sulfate, analytical grade reagent, no
further details
Remark:
Orobanche crenata Forsk. is a parasite weed.
Result: 4 mM ammonium sulfate reduced the germination % of O.
Crenata from about 50% in the controls to 16%. In the
presence of the nitrification inhibitor nitrapyrin, the
germination percentage of O. Crenata was reduced from c.55%
in the controls to 2%.
Test condition: TEST ORGANISMS: Orobanche crenata Forsk. seeds were
collected in Syria, at the Tel Hadya research station of the
Center for Research in Dry Areas (3600'N, 36056'E), and
stored in the dark at room temperature (15-250C) until use,
5-6 years later. The seeds were then surface-sterilised in
sodium hypochlorite solution (1% chlorine, wt/V) for 5
minutes, and then thoroughly rinsed with distilled water.
This treatment prevented fungal contamination to a large
extent.
TEST VESSEL: Glassware, including 5 cm Petri dishes, and
filters used in the experiment were sterile. Distilled water
was used to prepare the solutions.
TEST METHODOLOGY: 25-30 seeds chosen at random were evenly
spread on a 1 cm diameter Whatman GF/C glass fibre filter.
Three of these 1 cm filters with seeds were placed on a 4.7
cm diameter GF/C filter located in a 5 cm diameter Petri
dish, to which was added 2 ml of 0.3 mM pH 7 Hepes buffer
[N-(hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)].
There were 4 replicate Petri dishes for each concentration
used in this experiment. For conditioning, the Petri dishes
were then sealed, wrapped individually in aluminium foil,
and kept at 20 + 1 0C for 14 days. After conditioning, the
GF/C filters with seeds were taken out of the Petri dishes
and briefly (c. 30 seconds) allowed to dry. The filters with
seeds were then transferred to another Petri dish, to which
2 ml of a stimulating solution was added. This solution
contained 1 mg/l of the synthetic germination stimulant GR24
(see original paper for details) and 0.1% v/v acetone in the
different test solutions (including water alone, Hepes
buffer alone, buffer + 8 mg/l nitrapyrin, 4 mM (NH4)2S04,
and 4 mM (NH4)2S04 + nitrapyrin. At 7 days after the
addition of the stimulating solution, the germination
percentages (and also the lengths of the germ tubes) were
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