AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
processes.
Statistical analysis: two-way analysis of variance.
Reliability: (4) not assignable
no information on purity
10-APR-2006 (128) (129)
Type:
Sub-acute
Species: guinea pig Sex: male
Strain:
Hartley
Route of administration: inhalation
Exposure period:
5 or 20 days
Frequency of treatment: 6 hours/day, 5 days/week
Post exposure period: no
Doses: 1 mg/m3 (MMAD 0.4 um, geometric standard deviation 2.2)
Control Group:
other: filtered air
Method:
GLP:
Test substance:
Remark:
Result:
Test condition:
182
other: see Test Condition
no data
other TS: ammonium sulfate, reagent grade
In this study, animals with elastase-induced pulmonary
emphysema and saline-treated controls were used to determine
if lung function changes related to ammonium sulfate exposure
were greater (compared with controls) in animals with
experimentally induced emphysema.
Results from 29 treated and 27 control animals are reported.
No information is provided on mortality.
Body weight was not affected by ammonium sulfate exposure or
elastase treatment. Ammonium sulfate exposures had no
significant effect on any of the measured lung volumes, carbon
monoxide diffusion capacity or on quasistatic compliance.
There were no consistent differences observed between 5- and
20-day exposed animals.
The test animals (from Charles River Breeding Labs,
Kingston, Mass.) were isolated for 3 weeks after delivery.
Following isolation, animals were assigned to exposure
groups such that preexposure body weight distributions were
the same for all experimental groups exposed at the same
time.
Anesthetized Animals were instilled intratracheally with
either sterile saline or porcine pancreatic elastase (EO127,
Sigma Chemical Co.; 5 units/100 g bw). Elastase activity was
determined immediately before daily instillations by the
method of Sachar et al. (1955, Proc. Soc. Exp. Biol. Med. 90,
323-326), using elastin-orcein as
substrate. The doses of elastase were selected as the
maximum doses tolerated that produced less than 10% acute
mortality, based on results of previous studies (Busch et
al., 1984, Environ. Res. 33, 473-496).
Three weeks following intratracheal instillations, the then
approximately 10 week old guinea pigs were exposed in
Hazleton Systems Model 1000 stainless steel chambers for 5
or 20 days to filtered room air, 1 mg/m3 ammonium sulfate or 1
mg/m3 ammonium nitrate aerosols.
Chamber aerosol concentration was monitored continuously and
the variations throughout the chamber were less than 5% of the
mean concentration. Aerosol concentrations were
determined from four daily, 90-min, filter-pad samples;
ammonium sulfate concentrations were determined by an
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