AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no data
LC50: = 2000
Method:
other: see Test Condition
GLP:
no data
Test substance: other TS: ammonium sulfate, purity 99%
Result:
The LC50 was determined as 2000 mg/.
Test condition: The 96 hour median LC50 was estimated following trimmed
Spearman Karber method with 5% trimming (Hamilton et al.,
1977) and 24 hour renewal bioassay system prior to
commencement of the experiments to the experiments
investigating the changes in the respiratory epithelium. No
further experimental details are reported on the LC50
experiments.
For the investigations on the respiratory epithelium, 5
groups of ten fish each were exposed to 50 L of 200 mg/L of
ammonium sulfate prepared in tap water with 6 mg/L dissolved
oxygen at pH 7.5 and a temperature of 26 +/- 2 °C. Parallel
controls under similar conditions but without the addition
of ammonim sulfate were also maintained. Media were renewed
after every 24 hours. Feeding was allowed for a period of 2
hours on every alternate day before the renewal of the
media.
Five experimental and five control fish were sacrificed
after 5, 10, 20, 30 and 45 days of exposure and 1 cm long
fragments of the accessory repiratory organ were fixed and 6
micron thick paraffin sections were stained with Ehrlich´s
hematoxylin/eosin, and with various histochemical methods
for different carbohydrate moieties.
Reliability: (2) valid with restrictions
limited documentation of results
23-AUG-2005 (56)
Type:
static
Species:
Alburnus alburnus (Fish, estuary)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: = 310
Limit Test: no
Method:
other: see Test Condition
Year: 1979
GLP:
no
Test substance: other TS: ammonium sulfate, purity 99.5%
Result: Result: 96-hour LC50 = 310 mg/L (95% confidence limits: 274,
347 mg/L).
Test condition: Fish ( about 8 cm from Baltic Sea) were not fed during the
test period. The tests were carried out in at least six
concentrations and one control, with 10 fish at each
concentration. Fish were acclimated for at least two weeks
prior to the experiments and fed once a day until 48 hours
prior to the tests.
Water (salinity 7 o/oo) for the fishwas maintained at pH 7.8
(not observed during the test) and 10 °C. The fish were tested
in 60-liter of brackish water in 70-liter aquaria.
Reliability: (2) valid with restrictions
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