AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
limited documentation
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (57) (58)
Type:
flow through
Species:
other: Sciaenops ocellatus (Red Drum, a warmwater marine
fish), early life stage test
Exposure period: 10 day(s)
Unit:
Analytical monitoring: yes
LC50: = 27
Limit Test: no
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, no further details
Remark:
1. Nominal concentrations of ammonium sulfate were used as
the source of ammonium and ammonia in the experiments. The
results reported as ammonia concentrations have been
calculated from the total ammoniacal N using the formulas of
Whitfield as modified by Bower and Bidwell (1979). (See:
C.E. Bower and J.P. Bidwell (1979). Ionization of ammonia in
seawater: effects of temperature, pH, and salinity. Journal
of the Fisheries Research Board of Canada 35, 1012-1016.)
The LC50 value reported here has been back-converted, using
linearly extrapolated conversion ratios derived from the
nearest available tabulated entries for total ammoniacal
nitrogen and ammonia, and not from a back conversion using
the equations of Bower and Bidwell. The error caused by this
procedure is less than 4%.
2. Once lethal concentrations were established for eggs and
first-feeding larvae, three week old postlarvae were
evaluated for 1 week for changes in ammonia toxicity with
age and development. Post larvae were less sensitive than
newly hatched larvae. Whereas a concentration of 7.7 mg N
L-1 (calculated 0.55 mg/L ammonia) killed all newly hatched
larvae within 1 week, a slightly higher exposure was
tolerated by 3 week old fish.
Result: The 96 hour LC50 (95% confidence limits) was 5.6 (4.2 - 7.4)
mg/L total N (approximately converted from 0.39 (0.29 -
0.53) mg ammonia per litre, as calculated by the method of
Bower and Bidwell.)
Test condition: TEST ORGANISMS: Eggs were obtained from laboratory spawnings
induced by manipulations of photoperiod and temperature
cycles. Fertilised red drum eggs were exposed to controlled
concentrations of ammonium sulfate in static tests that were
maintained for up to 14 days. Culture methods are described
in Holt et al (1981). (Holt, J., Godbout, R., and C. R.
Arnold (1991) Effects of temperature and salinity on egg
hatching and larval survival of red drum Sciaenops
ocellatus. United States National Marine Fisheries Service
Fishery Bulletin 79, 569-573.)
TEST VESSEL: The test was a static test.PHYSICAL/CHEMICAL
PARAMETERS: The pH of the seawater used varied from 8.0 to
8.2. Salinity varied from 2.8 to 3.2%. Background
concentrations of un-ionized ammonia were 0.001-0.018 mg/L.
deg C temperature was maintained at either 25 or 26 deg C
+/- 0.5 0C. Dissolved oxygen varied from 5.4 to 6.4 mg/L.
TEST CONDITION: Three replicates of each concentration and
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