AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
group was given one mC of radioiodine I-131 in four equal
instalments of 250 uCi at three monthly intervals for one
year.
Another group was kept in 0.01 % ammonium sulfate for one
year. Water was changed three times a week.
The third group was kept in 0.03 % thiourea for three
months. The water was changed three times a week.
All fish were sacrificed by decapitation, and the adrenal
and thyroid were fixed in Bouin`s sublimate. As the thyroid
is in the form of scattered follicles in the region of the
ventral aorta and its afferent branchial arches, the entire
pharyngeal region was fixed. The picric acid of the fixative
was found to be sufficient to decalcify the pharyngeal bones
within a week. Paraffin sections were cut 3 and 5 um thick.
The thyroid and adrenal were stained in periodic acid Schiff
reagent (PAS)-haematoxylin and haematoxylin-eosin. The
pituary was stained in PAS-lead haematoxylin (PbH).
All experiments were run with equal numbers of controls.
Reliability: (2) valid with restrictions
basic data are given
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (88)
Species:
other: Channa punctatus
Endpoint:
other: ovarian changes
Exposure period: 6 month
Unit: mg/l Analytical monitoring: no
LOEC: = 100
Method:
GLP:
Test substance:
other: see Test Condition
no
other TS: ammonium sulfate fertilizer, not further specified
Remark:
Maturation of oocytes through stages I,II and III was
inhibited in the exposed fish. In stage III oocytes, the
nucleus exhibited degenerative changes.
Result:
No external manifestation of toxicity was observed, but
ovarian growth was significantly reduced (gonadosomatic
index of 1.13 vs 1.80 for the control).
Test condition: Ten adult C. punctatus purchased from a local fish market
were exposed in a 40-liter aquarium to 100 ppm ammonium
sulfate for six months. Ten additional fish were placed in a
second aquarium and served as a control. Dilution water was
well water with pH 7.2. Aquaria were maintained under
natural light and temperature such that the average monthly
water temperature from January to June was 20, 23, 27, 31,
32 and 35 °C, respectively. The water was changed every
alternate day after feeding the fish with goat liver. All
fish were sacrificed at the end of 6 months and the
histology of the ovaries was examined.
The experiments were ran from January when the ovary was in
the resting phase, and ended the last week of June when
control fish exhibited spawning phase ovaries with matured
vitellogenic stage-IV oocytes.
Reliability: (2) valid with restrictions
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (89)
UNEP PUBLICATIONS 125