AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, from Sigma Chemical company
Remark:
Result:
Test condition:
108
1) The ammonium sulfate concentrations studied here are much
higher than those which would be applied in the field. Thus
ammonium sulfate addition is not a practical way to control
S. Mansoni infestation.2) The LC5, LC50, and LC95 values
given below are as reported. However, the 6 hour values are
effected by mortality in the controls, due to the aging
population, and several of the other values given are
outside the range of the test concentrations used (0, 0.1,
0.2, 0.3, 0.4, and 0.5% ammonium sulfate.) Thus only the 4
hour LC50 value of 0.16% is recommended.
A 1% solution of ammonium sulfate was sufficient to
completely inhibit hatching of S. Mansoni eggs. The LC5,
LC50, and LC95 values for miracidial survival were 0.07%,
0.80%, and 10.61% after 2 hours of exposure; 0.03%, 0.16%,
and 0.90% after 4 hours of exposure, and 0.30, 0.20, and
0.40% after 6 hours of exposure.
The 4-hr LC50 was determined as 0.16% ammonium sulfate
TEST ORGANISMS: The life cycle of a Puerto-Rican strain of
Schistosoma mansoni has been maintained in the
Schistosomiasis Labotatory of the Tulane University School
of Public Health and Tropical Medicine, using Biomphalaria
glabrata, NIH albino strain, as intermediate hosts and
laboratory mice as definitive hosts. Schistosome eggs and
miracidia were obtained from the livers of infected mice,
which were removed by disection and homogenised at a low
speed for 20-30 seconds using a Waring blender. The
homogenate was poured into a flask for 20-30 minutes
settlement, after which the supernatant was carefully poured
off. The egg-containing sediment was used in the
hatchability and survival studies.
HATCHABILITY STUDIES: After resuspending and thoroughly
mixing the egg-containing sediment in 8-10 ml of 0.85% NaCl,
1.0 ml aliquots were transferred to each of several 100 ml
volumetric flasks which were then filled with the specified
dilutions of ammonium sulfate. Three replicates were made
for each ammonium sulfate concentration. The flasks, except
for the necks, were wrapped with aluminium foil and were
then exposed to light. The negatively geotactic and the
positively photoactic behaviour of the miracidia caused them
to aggregate in the necks. Thirty minutes later, the upper 5
ml were removed from the neck of each flask, and all the
miracidia present were killed with iodine and counted. The
percentages hatching were obtained by comparing the number
of miracidia obtained in each test concentration to that
obtained in the control with no added ammonium sulfate.
SURVIVAL STUDIES: The egg-containing sediment obtained from
the homogenate was rinsed into a 1 l sidearm flask,
previously covered, except for the sidearm, with black
cloth. The flask was then filled with dechlorinated water. A
bright light was nest directed toward the uncovered portion
of the flask. The negatively geotactic and the positively
photoactic behaviour of the miracidia caused them to
aggregate in the sidearm, after about 15 minutes. They were
then removed from the sidearm with a pasteur pipette and
transferred to wells of the test chamber, a laboratory
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