AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
- Morphometric analyses were performed on lungs of rats
exposed to purified air or to the high concentration, and
included measurements of the thickness and cellularity of the
alveolar wall, alveolar chord lengths and alveolar
cross-sectional diameters.
- Macrophage function analysis: Bronchoalveolar lavage (BAL)
was performed on 12 rats per group to obtain macrophages for
immunological testing and proteins for assessment of
epithelial permeability. Changes in functional characteristics
of alveolar macrophages were quantified by a rosette assay for
determining Fc receptor binding capacity for sheep red blood
cells, and by determination of the production of superoxide
anion during respiratory burst activity.
- Changes in airway permeability: permeability was determined
by measurement of albumin concentrations in the BAL fluid.
- Further exploratory endpoints that were measured were:
determination of the numbers of goblet cells in the
respiratory epithelium, releases of the inflammatory mediators
prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), and
measurements of respiratory frequency and tidal volume during
exposure.
STATISTICAL METHOD: one-way or two-way analyses of variance,
as appropriate. Tukey multiple comparison test for differences
between group means. The criterion for statistical
significance was p = 0.05.
Reliability: (3) invalid
Insufficient and partly contradictory documentation. The
experiments were performed in separate sets of exposures, with
separate control groups demonstrating a high variability in
results. Morphometric analyses were performed in only one
dose-group, and the relevance of the subtle changes reported
remains therefore unclear. The findings relating to macrophage
function were not dose related, and appear to be within the
normal physiological ranges. Unacceptable reporting.
21-JUN-2004 (133)
Type:
Sub-chronic
Species: rat Sex: male/female
Strain: Fischer 344
Route of administration: oral feed
Exposure period:
13 weeks
Frequency of treatment: continuously
Post exposure period: no
Doses: 0, 0.38, 0.75, 1.5, 3% in diet (corresponding to 0,
222, 441, 886, 1792 mg/kg bw/day in males and to 0,
239, 484, 961, 1975 mg/kg bw/day in females).
Control Group:
yes, concurrent no treatment
NOAEL:
= 886 mg/kg bw
NOAEL, females :
= 1975 mg/kg bw
Method:
GLP:
Test substance:
Result:
other: see Test Condition
no data
other TS: ammonium sulfate, purity not stated
BODY WEIGHTS: at study end final body weights were in males
298, 273, 287, 282 and 284 g for the 0, 0.38, 0.75, 1.5 and
3% groups, respectively. In females, final body weights were
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