AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
PHYSICAL/CHEMICAL PARAMETERS:
Determination of the temperature, dissolved oxygen and pH of
each test solution were made in conjunction with the daily
biological observation. The test temperature target was 20 +/-
1 °C. If the dissolved oxygen concentration in a test chamber
fell below 40% of the starting level in a test, the test was
repeated with 0.05 L/min glass-sparger aeration All tests were
conducted within the extremes of 6.5 to 8.5 pH units. The
photoperiod duration was 16 h of light. The air-water
interface of each tank received approximately 50 ft-c of
cool-white fluorescent light.
BIOLOGICAL PARAMETERS: biological observations were made
daily. Survival, condition and behavioural information were
recorded. Dead organism were removed when observed. If more
than one-half of the population exposed in any treatment was
found to be dead, additional aquaria containing lower
concentrations of test soution were set up.
EXPOSURE PERIOD: 96 hours.
TEST CONCENTRATION: 100, 10, 1 and 0.1 mg/l.
STATISTICAL METHOD: The 96-h LC50 value was derived using an
interpolation method as described by Stephan (Stephan CE:
Methods for calculating an LC50: Aquatic toxicology and
hazard evaluation. In: FL Mayer and JL Hamelink, eds., STP
634. American Society for Testing and Materials,
Philadelphia, PA, pp. 65-84). The linear interpolation uses
the logarithm transformation of the concentration and the
angle transformation of the percent dead between the two
doses that bracket 50% response.
YEAR OF STUDY: not reported.
Reliability: (2) valid with restrictions
concentration not measured. Limited documentation of
results.
Flag:
Critical study for SIDS endpoint
26-JAN-2004 (59)
Type:
static
Species:
other: Perna Viridis (green mussel, seawater)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
LC50 : = 94.9
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, reagent-grade, no further details
Remark: The pH variation over the experiment, from pH 8.125 at 5
mg/L NH4+ to pH 7.85 at 75 mg/L NH4+ is small, and a
slightly smaller percentage of unionised ammonia will be
present at the higher concentration level. Thus the
experiment will correctly reflect the toxicity of the
ionised ammonium salt.
Result:
The 96 hour LC50 was 94.9 mg ammonium sulfate N/l
(converted from 13.0 mg NH4+ L-1); 5% confidence limit 12.5 -
14.0 mg/NH4/l.
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