AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Test condition:
Test substance:
mice treated with sulfate. No differences were found in
litter size, litter weaning weights, or gestational or
lactational weight gain of the dams among sulfate
treatments.
60 random-bred ICR female virgin mice were used. Mice were
assigned randomly to one of six water treatments (control,
Na control, 4 dose levels of sodium sulfate). All groups
contained equal levels of Na, which was maintained by
varying the amount of sodium bicarbonate added. The treated
water was available ad libitum beginning 1 wk prior to
breeding and was continued throughout the experiment.
After 1 wk of acclimation to the treatments, a male mouse
that had received tap water was paired randomly with each
female mouse. The females were checked every 24 hours in the
morning for the presence of a vaginal plug. After a vaginal
plug was observed, the male mouse was removed and the female
was weighed.
Water consumption was measured daily during the 2nd and 3rd
wk of gestation, and the 1st and 2nd wk of lactation. At
parturition, the dams were weighed and litter size was
recorded. The litters then were standardized to eight pups
per litter. At 21 days postpartum, the pups were weaned and
the litters and dams were weighed individually. The dams
were then rebred at first estrus immediately following
weaning. This procedure was carried out over two parities.
Only animals that whelped during each parity were used in
the analysis. Thus, the number of dams per dose group in the
first parity was 4-9, in the second parity it was only 4.
Fertility indices were not measured.
Statistical analysis: least squares mean analysis of
variance (SAS, 1982). Student`s t-test was used to determine
the difference in water consumption.
The doses 625, 1250, 250 and 5000 mg sulfate/ litre
corresponding to:
625 mg sulfate/l:
first parity
gestation: ca. 250-300 mg sulfate/kg bw
lactation: ca. 450-710 mg sulfate/kg bw
second parity
gestation: ca. 270-360 mg sulfate/kg bw
lactation: ca. 470-840 mg sulfate/kg bw
1250 mg sulfate/l:
first parity
gestation: ca. 480-570 mg sulfate/kg bw
lactation: ca. 930-1450 mg sulfate/kg bw
second parity
gestation: ca. 595-920 mg sulfate/kg bw
lactation: ca. 1390-2040 mg sulfate/kg bw
2500 mg/kg sulfate/l:
first parity
gestation: ca. 1270-1660 mg sulfate/kg bw
lactation: ca. 2290-3180 mg sulfate/kg bw
second parity
gestation: ca. 1500-1875 mg sulfate/kg bw
lactation: ca. 2560-4320 mg sulfate/kg bw
5000 mg sulfate/l
198
UNEP PUBLICATIONS