AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
toxic to Testacea in alpine pasture.
Test condition: Three sites of alpine pasture were treated with 100, 400,
and 1200 kg/ha Ammonium sulfate on 13 June. Nematodes,
Testacea, and Ciliates were collected from each site and
from the control site on July 5, Sept 5, and Oct 18.
Testacea biomass was unaffected at the lower ammoniumsulfate
levels, but increased by a factor of 1.4 at the highest
level.
Reliability: (4) not assignable
Abstract of conference presentation. Limited documentation.
Pilot study.
25-APR-2003 (98)
4.6.4 Toxicity to other Non-Mamm. Terrestrial Species
Species:
Expos. period:
Method:
GLP:
Test substance:
Remark:
Result:
Test condition:
other: Pseudacris regilla (Pacific treefrog)
10 day(s)
other: see Test Condition
no data
other TS: ammonium sulfate, 99.2% pure (Reagent Grade,
Mallinckrodt Baker Inc, Phillipsburg, NJ)
These tadpoles are from different egg masses than those used
for the 9 week-old test (see following IUCLID entry).
The NOEC for the tadpoles, which were 6 weeks old at the
start of the 10 day test, was 17.4 mg/L ammonium nitrogen.
The corresponding LOEC was 37.0 mg/L ammonium nitrogen.
TEST ORGANISMS: Eggs of Pseudacris regilla were collected
locally from non-agricultural areas and hatched in the EPA
laboratory facilities at Corvallis, Ore. Tadpoles were
subsequently reared in aquaria with a continuous flow of
temperature controlled fresh water whose temperature was
gradually increased from the collection temperature of ca.
10 deg C to the test temperature of 20 +/- 1 deg C.
Temperature was continuously recorded. They were held at a
photoperiod of 14:10 light:dark. Newly hatched Pseudacris
regilla were fed pelleted rabit feed, with small amounts of
newly hatched brine shrimp and then frozen fish food.
TEST VESSEL: The test species were exposed to ammonium
sulfate in 18 10-L aquaria with a continuously-flowing water
diluter system that automatically delivered six
concentrations of chemical and controls to three aquaria per
concentration.
PHYSICAL/CHEMICAL PARAMETERS: Rearing and test water was
obtained from wells near the Willamette River at Corvallis,
Oregon. Dissolved oxygen, measured by electrode, was
maintained near saturation by the flowing water. pH (median
value 7.3) was measured by electrode. Total hardness (72 +/-
4.2 mg/L), alkalinity (63 +/- 2.8 mg/L), and conductivity
(188.8 +/- 7.3 µS/cm) were measured by EPA methods 130.2,
310.1, and 120.1, respectively. Background ammonium-N ranged
from 0.005 - 0.010 mg/L in well water, and from 0.00 to 0.03
mg/L in control tanks. Water samples for measurement of
ammonium sulfate were taken from each concentration (each
aquarium in the diluter) on four days, and analysed with a
Hach DR/700 digital photometer. Quality assurance techniques
were used in the analysis.
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