AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
7.2; a dilution to 200 mOsm/kg H2O had no influence on pH.
Further dilution led to a pH decrease and medium of 55
mOsm/kg H2O had a pH of 5.9.
HYPOTONIC TREATMENT: Hypotonic treatment was carried out on
monolayer cells in petri dishes. The culture medium was
discarded, then the cells were washed with 5 mL of prewarmed
phosphate-buffered saline (PBS). Immediately afterwards, 5
mL of prewarmed salt solution or diluted McCoy´s medium was
added. After 30 minutes at 37 degr C, the salt solution or
the hypotonic medium was discarded, the cells were washed
once with PBS, and then freshly prepared culture medium with
BrdUrd was added. After a culture time of 16 hr, including a
2-hr colcemid treatment, the cells were fixed in
methanol:acetic acid and stained with 5% Giemsa or according
to the fluorescence plus Giemsa (FPG) technique. All
experiments were performed twice or three times without
BrdUrd and once with BrdUrd labeled cells.
Reliability: (3) invalid
Unsuitable test system (mostly unphysiological, hypotonic
test conditions; the isotonic exposure exceeded the maximum
recommended exposure level of 5 mg/mL).
12-APR-2006 (143)
System of testing:
Result:
E. coli
positive
Reliability: (3) invalid
Erroneously cited. Original publication (Demerec et al.,
1951) does not contain data relating to ammonium sulfate.
18-APR-2003 (11)
Type:
other: DNA synthesis
System of testing: nucleotides, nucleic acid synthetic analogue and DNA
polymerase from avian myeloblastosis virus
Concentration:
at least 7 concentrations between 20 um and 150 uM (not
specified)
Cytotoxic Concentration: not reported
Metabolic activation: without
Result:
negative
Method:
GLP:
Test substance:
Result:
Test condition:
Reliability:
other: see Test Condition
no data
other TS: ammonium sulfate, not specified further
In a screening experiment 31 salts were tested
for their ability to affect the accuracy of DNA synthesis
in vitro. A good correlation was found between known
metal carcinogens and the decrease in accuracy of DNA
synthesis. Ammonium sulfate had no effect on the precision
of DNA synthesis in this test system.
The influence of various salts on the accuracy of DNA
synthesis was investigated in a solution of nucleotides
containing a nucleic acid synthetic analogue. As matrix
virus DNA polymerase and 31 different salts were used.
(4) not assignable
insufficient information available to allow assessment of
reliability
UNEP PUBLICATIONS 191