AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
emergence of immature fry.
Test condition: Three types of tests were conducted:
(1) Eyed eggs, alevins, and fry were exposed in separate
tests to short term, high concentrations of ammonia (> 50
ppb) in static systems to determine the sensitivity of each
early life stage.
(2) Alevins were exposed at different developmental stages
to low concentrations of ammonia (< 3 ppb) in flow-through
systems for up to 61 days to determine the effect of
long-term exposures on size of emerging fry.
(3) Alevins were exposed to high concentrations of ammonia
(30-150 ppb) in flow-through systems for 24 hours to
determine whether ammonia would cause emergence of immature
fry.
Eggs, alevins, and fry were also exposed to static solutions
of ammonium sulfate for 96 hours in freshwater at pH of
6.3-6.5 and 3.7-4.8 °C. Each test group consisted of 25
individuals.
Reliability: (2) valid with restrictions
limited documentation
02-FEB-2004 (28)
Type:
static
Species:
Pimephales promelas (Fish, fresh water)
Exposure period: 10 day(s)
Unit: mg/l Analytical monitoring:
Method:
other
GLP:
no data
Test substance: as prescribed by 1.1 - 1.4
Result:
Test condition:
92
The NOEC for the juvenile fish, which were 6 weeks old at the
start of the 10 day test, was 66.6 mg/L ammonium
nitrogen. The corresponding LOEC was 118.0 mg/L ammonium
nitrogen.
The NOEC for the juvenile fish, which were 9 weeks old at
the start of the 10 day test, was 134.0 mg/L ammonium
nitrogen. The corresponding LOEC was 227.0 mg/L ammonium
nitrogen.
TEST ORGANISMS: Pimephales promelas were hatched and reared
in a laboratory colony in the EPA laboratory facilities at
Corvallis, Ore. They were reared in aquaria with a
continuous flow of temperature controlled fresh water whose
temperature was gradually increased from the collection
temperature of ca. 10 0C to the test temperature of 20 +/-
1 deg C. Temperature was continuously recorded. They were
held at a photoperiod of 14:10 light:dark. The fish were fed
newly hatched brine shrimp and then frozen fish food.
TEST VESSEL: The test species were exposed to ammonium
sulfate in 18 10-L aquaria with a continuously-flowing water
diluter system that automatically delivered six
concentrations of chemical and controls to three aquaria per
concentration.
PHYSICAL/CHEMICAL PARAMETERS: Rearing and test water was
obtained from wells near the Willamette River at Corvallis,
Oregon. Dissolved oxygen, measured by electrode, was
maintained near saturation by the flowing water. pH (median
value 7.3) was measured by electrode. Total hardness (72 +
4.2 mg/L), alkalinity (63 + 2.8 mg/L), and conductivity
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