AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
results.
Harlan Sprague Dawley (Indianapolis, Ind.). were acclimated
for 1 week at 21 °C, 50% humidity, and a 12:12 h light/dark
cycle. The mean initial body weight was 23.0 +/- 1.7g. Mice
were housed in groups of five, and maintained on commercial
rodent chow (Harlan Teklad 22/5 rodent diet, Harlan Teklad,
Madison, Wis.).
EXPOSURE TO TEST SUBSTANCE: Mice were administered aluminum
ammonium sulfate in deionized drinking water ad lib at
levels of 0, 5, 25, and 125 ppm as aluminum for 1 month.
Controls received deionized water. An additional group was
administered 0.611 g/L of ammonium sulfate to provide an
ammonium ion concentration of 250 ppm. The results of this
additional group were compared with those of the control to
determine whether ammonium or sulfate ions affected the
EXAMINATIONS: The weights of mice were recorded weekly and
water
consumption measured daily. Animals were observed daily for
general behavior such as appearance, activity, grooming, and
locomotion. On the last day of treatment, brains were
isolated and the cerebrum was dissected from brain. A
representative sample of whole cerebrum was employed to
extract RNA. RNA was quantified by spectrophotometric
absorbance at 260 nm. In PCR amplified samples, the
following cytokines were determined: TNF-alpha, IL-1beta,
and IFN-gamma. beta-actin from cDNA was also amplified and
served as internal standard. Splenic macrophages and
lymphocytes were collected according to the method
previously described by Sharma, 1996. Liver, kidney and spleen
were weighed.
STATISTICAL EVALUATION:
The log-transformed data of control and treatment groups
were compared by one-way analysis of variance (ANOVA)
followed by Fisher`s post-hoc least significant difference
(PLSD) test using the Statview software (Abacus Concept,
Berkeley, Calif.). For statistical analyses different
treatment groups were compared to the group given deionized
water.
YEAR of STUDY CONDUCT: not reported.
Reliability: (2) valid with restrictions
limited documentation, no guideline study, no histopathology,
only three organ weights determined, only one dose tested
10-APR-2006 (135)
Type:
Sub-acute
Species: rabbit Sex: female
Strain:
no data
Route of administration: oral unspecified
Exposure period:
5-16 months
Frequency of treatment: on alternate days
Post exposure period: no
Method:
GLP:
Test substance:
other: see Test Condition
no
other TS: ammonium sulfate, not further specified
Result: At necropsy, mean body weights were between 4,100 and 4,800
grams. Parathyroid weights were between 15 and 33 mg (mean
value 27.5 mg). Parathyroids of control animals (n=50)
178
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