AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Type:
LD100
Species:
rat
Strain:
Wistar
Sex:
male
Route of admin.: i.p.
Test substance:
other TS: ammonium sulfate, reagent grade
Result:
The effect of ammonia intoxication has been studied after
intraperitoneal injection of various ammonium salts to rats
including ammonium sulfate. A standardized dose of
10.6 mmol NH4-N/kg (1,4 g/kg bw) was lethal within 8-13
minutes for all 13 ammonium salts tested including ammonium
sulfate.
Reliability: (2) valid with restrictions
limited documentation. No further information on purity.
18-NOV-2003 (109)
Type:
other: pulmonary oedema
Species:
rat
Strain:
Wistar
Sex:
male/female
No. of Animals: 19
Doses:
6 % (600 mg/kg bw)
Route of admin.: i.p.
Method:
other
Year: 1969
GLP:
no data
Test substance: other TS: ammonium sulfate
Result:
Test condition:
160
The major lesion was the formation of large blebs in
endothelial cells, associated with a variable degree of oedema
of the interstitium and of epithelial lining cells. These
changes were present in every animal exposed to ammonium
sulfate, irrespective of the time-interval after injection,
the only difference being that the changes prominent in those
exposed for longer intervals.
The ultrastructural changes, produced by ammonium sulphate are
similar to the changes in pulmonary oedema induced by methods
other than by altered haemodynamics. The absence of
carbon-marker leakage through intercellular junctions in this
experiment suggest that the increased vascular permeability
necessary for the formation of pulmonary oedema differs from
leakage occurring in acute inflammation, in quality if not in
mechanism.
19 rats were used. A colloidal carbon suspension was injected
into the tailveins of the rats in a dosage of 0.1 ml per 100 g
bw. immediately after, a 6 % solution of ammonium sulfate was
injected i.p. in a dosage of 10 ml/kg bw. Rats were killed at
varying intervals (1.5, 5, 7.5, 10, 15, 20, 30, 90 min) after
injection of ammonium sulfate. 9 control animals were used.
The lungs were prepared and examined by electron microscopes.
Other organs like liver, spleen, kidney, heart and skeletal
muscle were prepared and stained as a routine with
haematoxylin and eosin, and in some instances with Weigert´s
elastic tissue stain or by the periodic acid-Schiff method and
examined microscopically or stained with uranyl acetate and
examined by electron microscopy.
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