AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
solutions (200, 150, 75 mOsm); corresponding to about
100; 66.6; 50; 25 mM ammonium sulfate (= 13.2; 8.8;
6.6; 3.3 mg/mL)
Cytotoxic Concentration: treatment with less than 30 mOsm/kg H20 was cytotoxic
Metabolic activation: without
Method:
other: see Test Condition
GLP:
no data
Test substance: other TS: ammonium sulfate, purity 99.5%
Remark:
Result:
Test condition:
The study was designed to investigate the effect of
hypotonic solution on the induction of chromosomal
aberrations.
The reported value of 7.5 % aberrant cells for the 300 mOsm
dose level fell well within the laboratory´s historical
control range and was hence considered a negative result
(personal communication).
Hypotonic solutions of the culture medium alone or from
chemicals including ammonium sulfate revealed an increase of
chromosomal aberrations. Ammonium sulfate mostly induced
chromatid type aberrations. Treatment with less than
30 mOsm/kg H2O was cytotoxic. Reasons for the aberrations
observed in hypotonic media may be a directly induced DNA
damage such as double strand breaks or a release of DNAse
after lysosomal damage because of the hypotonic treatment.
Other reasons involved in the induction of aberration
production may be changes of the internal pH or damage of
the chromosomal proteins.
% ABERRANT METAPHASES (100-300 metaphases analyzed, fixation
time 15 hours):
1.0 %, 7.5%, 19.0%, 36.6% and 60.3% for controls, 300, 200,
150 and 75 mOsm ammonium sulfate, respectively.
YEAR OF STUDY: not reported.
TEST SYSTEM: V79 hamster cells were grown at 37 deg C in a
humidified atmosphere with 5% CO2 in McCoy`s 5A medium,
supplemented with 10% fetal calf serum, 100 units
penicillin, and 0.128 mg/mL dihydrostreptomycin sulfate in
10-cm petri dishes; 48 hr before the treatment 10e5 cells
from the stock culture were seeded in 6-cm petri dishes with
5 mL of complete medium.
PREPARATION OF SALT SOLUTIONS AND MEDIUM: Ammonium sulfate,
sodium chloride and trishydroxymethylaminomethane, were
dissolved in distilled water; the stock solution was further
diluted until the appropriate osmolality was reached.
Undiluted McCoy`s medium had 300 mOsm/kg H2O. A dilution of
1:9 (medium:water) reduced the osmolality to 30 mOsm/kg H20.
For ammonium sulfate, sodium chloride, and trishydroxymethyl
aminomethane there is a clear correlation of molarity and
osmolality. The
molarity (mM) of ammonium sulfate solutions has to be
multiplied by a factor of 3 to give the osmolality (mOsm/kg
H20). The osmolality of all solutions was measured with a
microosmometer.
The pH of the ammonium sulfate and sodium chloride solutions
was 5.5 independent of the concentration. The pH of McCoy´s
medium changed with dilution. Undiluted medium had a pH of
190
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