AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Concentration:
3.2 M (423 mg/mL)
Cytotoxic Concentration: cytotoxicity not reported
Metabolic activation: without
Result:
negative
Method:
GLP:
Test substance:
Remark:
Result:
other: see Test Condition
no data
other TS: ammonium sulfate, analytical grade
The authors suggest that ammonium sulfate may induce
conformational changes in the chromatin which make more
recognition sites available for the Alu I enzyme.
The frequencies of chromosomal aberrations induced by the
restriction endonuclease Alu I (recognition site AG/CT)
could be elevated to a similar extent by additional
treatments with a single-strand-specific endonuclease from
Neurospora crassa, or with ammonium sulfate in which the
Neurospora endonuclease was suspended.
Effects of the restriction endonuclease Alu I on chromosomes
were more pronounced when the cells were treated
additionally with ammonium sulfate. The chromosomal
aberration rates in cells treated with ammonium sulfate
alone were not increased:
% ABERRANT METAPHASES (200 metaphases analyzed, 22 hr after
the treatment with 3.2 M ammonium sulfate for 15 min;
including achromatic lesions): 15.5% (control with
Neurospora crassa endonuclease instead of ammonium sulfate:
20%).
Test condition: Chinese hamster ovary cells (CHO) were grown in McCoy`s 5A
medium, supplemented with 10% fetal calf serum, 100 units
penicillin and 0.128 mg/mL dihydrostreptomycin sulfate.
4x10e6 cells from exponentially growing cultures were
centrifuged and repelleted in 1 mL newborn calf serum (NCS).
Alu I was prepared by mixing stock solution with NCS to give
a final volume of 8 uL which was added to the pellet.
Neurospora endonuclease or 3.2 M ammonium sulfate solution
in distilled water were added to the Alu I-treated cells at
different times. The treatments were finished 20 minutes
after addition to Alu I by washing the cells.
Controls included cells treated with medium + NCS (8 uL) and
5 minutes later with 10 uL 3.2 M ammonium sulfate or
neurospora endonuclease, added for 15 minutes. Cells were
fixed and stained 22 hours after treatment, including a
treatment with colcemid (0.08 ug/mL) for 2 hrs.
The following aberrations were analysed: achromatic lesions,
chromatid breaks, isochromatid/chromosome breaks, chromatid
intrachanges, chromatid interchanges, triradials,
polycentric chromosomes, ring chromosomes and minutes.
YEAR OF STUDY: not reported.
Reliability: (2) valid with restrictions
limited documentation; only one dose level studied, no
standard chromosome aberration test, no negative control,
however a very high concentration has been used.
10-APR-2006 (142)
Type:
System of testing:
Concentration:
Cytogenetic assay
V79 cells
osmotic solution of 300 mOsm/kg H20, and hypotonic salt
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