AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
automated methyl-thymol blue method. Particle-size
distribution was measured from combined Mercer cascade
impactor samples, collected over 15 hours of exposure during a
5-day week. Daily impactor samples were collected at a
constant flowrate of 2 liters/min on glass coverslips. Mass
median aerodynamic diameter (MMAD) and geometric standard
deviation (GSD) of the aerosol were calculated using a program
which compared the impactor data with a log-normal
distribution.
For the evaluation of pulmonary function, animals were
anesthetized by intraperitoneal injection with ethyl
carbamate and then intubated with an esophageal catheter and
placed prone in a flow-type plethysmograph. Transpulmonary
pressure (airway pressure minus esophageal pressure) was
determined by electronic subtraction of the two signals.
Before making any measurements, the lungs were inflated
twice to total lung capacity to establish a constant volume
history. Lung volumes in spontaneously breathing animals
were determined by connecting a syringe to the airway
opening, evacuating the lungs to residual volume, then
inflating the lungs to total lung capacity. During these
maneuvers, volume excursions and transpulmonary pressure
were recorded. Residual volume was determined by inert gas
dilution, using 0.5% neon in air as the test gas.
Single-breath carbon monoxide diffusion capacity of the lung
was determined by a modification of the technique of
Takezawa et al. (1980, J.App.Physiol. 48, 1052-1059).
Following these measurements, animals were paralyzed by
intramuscular injections of succinyl choline chloride and
artificially ventilated with a rodent respirator. Quasistatic
compliance and single-breath N2 washout maneuvers were
performed by inflating the lungs with air or O2, respectively
at flow rates of 2.5 mL/sec to twice the total lung capacity,
and then deflating the lungs, at the same flow rate, to
residual volume. Functional residual capacity (the volume of
gas in the lungs following normal passive exhalation) was
measured in paralyzed animals using the inert gas dilution
technique used to measure residual volumes in spontaneously
breathing animals.
Statistical Method: analysis of variance.
Year of study: not reported.
Reliability: (4) not assignable
limited documentation, no further characterization of purity
10-APR-2006 (132)
Species: other: donkey Sex:
Route of administration: inhalation
Exposure period:
about a year
Frequency of treatment: 1 hour weekly
Post exposure period: no data
Doses:
0.3 - 3.1 mg/m3 (MAD 0.3-0.6 um)
Control Group:
other: H2SO4 aerosol
Method:
GLP:
Test substance:
Result:
other: see Test Condition
no
other TS: (NH4)2SO4, purity not stated
Short -term slowing of clearance followed certain single
exposures to H2SO4 at 194-1364 ug/m3 (in three of four
UNEP PUBLICATIONS 183