AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
of the control contained an average of 65 eggs each. Eggs
hatched within 24 hours, and larvae began to feed on the
third day post hatch. Mortality was assessed daily.
Experiments with older fish included 3 to 4 replicates with
6 to 10 fish each. Concentrations were 0.2, 1.5, 3.6, 4.5,
7.7, 20.0, 100.0, and 500.0 total nitrogen, measured with a
Gilford spectrometer. Concentrations were adjusted
throughout the exposure to keep them within 10% of the test
doses.The 96 hour median lethal concentrations were
calculated by the method of Litchfield and Wilcoxon.
Treatment means were compared with the controls by Dunnetts
test with a 5% significance level.
Reliability: (1) valid without restriction
Flag:
Critical study for SIDS endpoint
23-AUG-2005 (27)
Type:
static
Species:
Pimephales promelas (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: > 100
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, reagent-grade, no further details
Remark:
In this study, 27 commercial inorganic and organic chemicals
were tested simultaneously in seven aquatic species.
Result:
A LC50 value could not be determined because the proportion
killed at the highest dose level (100 mg/L) was less than
50%. No further details reported.
Test condition: TEST ORGANISMS: Fathead minnow (pimephales promelas), 0.2 -
0.5 g. The test organisms were acclimated to the control
diluent water in the breeding/rearing tanks (time period not
reported). Food was withheld for the 24 hours preceding
start of the test. Juveniles as uniform in size as possible
were collected from the colonies. Ten juvenile organisms
were exposed to the test chemical in each treatment.
Biological loading was kept below 0.5 gram wet weight per
liter of test solution. The average wet weight of a randomly
chosen set of minnows was determined at the start of the test.
TEST VESSEL: The assay was performed in seamless glass,
30.5-cm cuboidal, Pyrex chromatographic jars to which 20
liters of test solution was added.
TEST SOLUTION: The test chemical was added directly to the
diluent water to give a nominal concentration of 100 mg/L.
The test chemical concentrations were not analyzed. Once the
test solutions were prepared, the starting temperature,
dissolved oxygen and pH values were determined for each
exposure concentration. When the starting pH of the test
soluton fell outside the extremes of 6.5 to 8.5, the pH was
adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%
(v/v) H2SO4.
DILUTION WATER: Water quality was routinely monitored to
characterize the diluent water and ensure its suitability
according to the standard method for the examination of
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