AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
+/- 16, 126.5 +/- 5, 81.5 +/- 5, 52.5 +/- 5, 32.4 +/- 3, and
0 mg/L ammonium nitrogen, respectively. Ten day LOEC values
and NOEC values were determined with the Dunnetts multiple
comparison procedure.
Reliability: (1) valid without restriction
Flag:
Critical study for SIDS endpoint
22-JUL-2004 (67)
Species:
Expos. period:
Method:
GLP:
Test substance:
Result:
Test condition:
other: Rana aurora (Redlegged frog)
10 day(s)
other: see Test Condition
no data
other TS: ammonium sulfate, 99.2% pure (Reagent Grade,
Mallinckrodt Baker Inc, Phillipsburg, NJ)
The NOEC for the tadpoles, which were 4 weeks old at the
start of the 10 day test, was 82.7 mg/L ammonium nitrogen.
The corresponding LOEC was 134.0 mg/L ammonium nitrogen.
TEST ORGANISMS: Eggs of Rana aurora were collected locally
from non-agricultural areas and hatched in the EPA
laboratory facilities at Corvallis, Ore. Tadpoles were
subsequently reared in aquaria with a continuous flow of
temperature controlled fresh water whose temperature was
gradually increased from the collection temperature of ca.
10 0C to the test temperature of 20 +/- 1 deg C.
Temperature was continuously recorded. They were held at a
photoperiod of 14:10 light:dark. Newly hatched Rana aurora
were fed pelleted rabit feed, with small amounts of newly
hatched brine shrimp and then frozen fish food.
TEST VESSEL: The test species were exposed to ammonium
sulfate in 18 10-L aquaria with a continuously-flowing water
diluter system that automatically delivered six
concentrations of chemical and controls to three aquaria per
concentration.
PHYSICAL/CHEMICAL PARAMETERS: Rearing and test water was
obtained from wells near the Willamette River at Corvallis,
Oregon. Dissolved oxygen, measured by electrode, was
maintained near saturation by the flowing water. pH (median
value 7.2) was measured by electrode. Total hardness (28.5
+/- 7.8 mg/L), alkalinity (30.0 +/- 7.1 mg/l), and
conductivity (87.0 +/- 17.8 µS/cm) were measured by EPA
methods 130.2, 310.1, and 120.1, respectively. Background
ammonium-N ranged from 0.005 - 0.010 mg/l in well water, and
from 0.00 to 0.03 mg/L in control tanks. Water samples for
measurement of ammonium sulfate were taken from each
concentration (each aquarium in the diluter) on two days,
and analysed with a Hach DR/700 digital photometer. Quality
assurance techniques were used in the analysis.
TEST CONDITION: Five 4-week old tadpoles were used in each
of three * replicate aquaria, and the test was run for 10
days. Length and mean wet wet were measured at the end of
the test. The means and standard deviations of the
ammonium-nitrogen concentrations measured at the 5
experimental concentrations and in the control were 227.0
+/- 1.4, 134.0 +/- 7.1, 82.7 +/- 3.2, 50.5 +/- 4.9, 28.8
+/- 5.2 and 0 mg/L ammonium nitrogen, respectively. Ten day
138
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