AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Test condition:
killed at the highest dose level (100 mg/L) was less than
50%. No further details reported.
TEST ORGANISMS: Snail (Helisoma trivolvis), 0.180 g. The
test organisms were acclimated to the control diluent water
in the breeding/rearing tanks (time period not reported).
Food was withheld for the 24 hours preceding start of the
test. Juveniles as uniform in size as possible were
collected from the colonies. Ten juvenile organisms were
exposed to the test chemical in each treatment. Biological
loading was kept below 0.5 gram wet weight per liter of test
solution.
TEST VESSEL: The assay was performed in seamless glass,
30.5-cm cuboidal, Pyrex chromatographic jars to which 20
liters of test solution was added. The test organisms were
placed directly in the aquaria.
TEST SOLUTION: The test chemical was added directly to the
diluent water to give a nominal concentration of 100 mg/L.
The test chemical concentrations were not analyzed. Once the
test solutions were prepared, the starting temperature,
dissolved oxygen and pH values were determined for each
exposure concentration. When the starting pH of the test
soluton fell outside the extremes of 6.5 to 8.5, the pH was
adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%
(v/v) H2SO4.
DILUTION WATER: Water quality was routinely monitored to
characterize the diluent water and ensure its suitability
according to the standard method for the examination of
water and wastewater, 16th ed., American Public Health
Association, Washington DC pp. 689-832. The chemical
characteristics are reported in the publication and are in
accordance with standards required by current testing
guidelines. Activated carbon-filtered, dechlorinated and
tempered industrial service water from Lake Ontario was used
in all tests.
PHYSICAL/CHEMICAL PARAMETERS:
Determination of the temperature, dissolved oxygen and pH of
each test solution were made in conjunction with the daily
biological observation. The test temperature target was 20 +/-
1 °C. If the dissolved oxygen concentration in a test chamber
fell below 40% of the starting level in a test, the test was
repeated with 0.05 L/min glass-sparger aeration All tests were
conducted within the extremes of 6.5 to 8.5 pH units. The
photoperiod duration was 16 h of light. The air-water
interface of each tank received approximately 50 ft-c of
cool-white fluorescent light.
BIOLOGICAL PARAMETERS: biological observations were made
daily. Survival, condition and behavioural information were
recorded. Dead organism were removed when observed. If more
than one-half of the population exposed in any treatment was
found to be dead, additional aquaria containing lower
concentrations of test soution were set up.
EXPOSURE PERIOD: 96 hours.
TEST CONCENTRATION: 100, 10, 1 and 0.1 mg/l
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