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AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

Method:<br />

GLP:<br />

Test substance:<br />

other: see Test Condition<br />

no<br />

other TS: ammonium sulfate, analytical grade<br />

Result:<br />

LC50 values reported for 24, 48, 72 hours were 430, 340, and<br />

290 mg/L, respectively.<br />

Test condition: The chemical was tested at different concentrations (60, 80,<br />

100, 1<strong>20</strong>, 150, 170, 190, 240, 300 ppm), selected from the<br />

results of a preliminary test. In each concentration, 10<br />

fishes were kept and were observed at short intervals during<br />

the first day. Dead specimens were removed as soon as<br />

possible.<br />

Every 24 hours, all remaining fishes in an aquarium were<br />

carefully transferred to a freshly prepared solution.<br />

Temperature and pH were regularly measured and samples from<br />

the test aquaria were taken at intervals for chemical<br />

analysis.<br />

Reliability: (2) valid with restrictions<br />

limited documentation<br />

Flag:<br />

Critical study for SIDS endpoint<br />

26-JAN-<strong>20</strong>04 (53) (54)<br />

Type:<br />

semistatic<br />

Species:<br />

Lebistes reticulatus (Fish, fresh water)<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: no<br />

LC50: = 592<br />

Limit Test: no<br />

Method:<br />

other: see Test Condition<br />

Year: 1987<br />

GLP:<br />

no data<br />

Test substance: other TS: ammonium sulfate, not further specified<br />

Remark:<br />

1 ppm was converted to 1 mg/l as no other information was<br />

presented.<br />

Result:<br />

No mortality occurred at 300 ppm even after 7 days. Complete<br />

mortality occurred at 900 ppm. Toxicity symptoms included a<br />

high metabolic rate, reddish gills due to hemorrhage, and<br />

sluggish movement. The toxicit of ammonium sulfate depends on<br />

the concentration of free un-ionized ammonia, which tends to<br />

increase in alkaline medium.<br />

No change towards increased toxicity was observed with<br />

prolonged exposure times.<br />

Test condition: Fish (mature and gravid females) were acclimated for 10 days<br />

in 10-L glass aquaria at pH 8.1-8.4 and 18.5-23.5 °C. Fish<br />

were exposed at <strong>20</strong> °C to<br />

ascending concentrations of ammonium sulfate (300 to 900<br />

ppm) in batches of 10. Solutions were renewed on alternate<br />

days.<br />

Mortality, defined as median tolerance limits (TLm), were<br />

determined at 24, 48, 72 and 96 hours.<br />

Reliability: (2) valid with restrictions<br />

limited documentation<br />

Flag:<br />

Critical study for SIDS endpoint<br />

26-JAN-<strong>20</strong>04 (55)<br />

Type:<br />

Species:<br />

semistatic<br />

other: Heteropneustes fossilis<br />

UNEP PUBLICATIONS 81

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