AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
LC50 values increased from 0.031 to 0.111 mg un-ionized
NH3-N/L at mean pH 6.0 as temperature increased from 2.1 to
17.1 °C.
Similarly, at mean pH 6.45, mean LC50 increaed from 0.030 to
0.146 mg NH3-N/L as temperature increased from 1.8 to 12.5°C.
The behaviour of intoxicated fish consisted of coughing,
hyperventilation followed by sporadic ventilation, twisting,
loss of equilibrium and spiral swimming, convulsions and death
following a coma-like state.
Test condition: The fish were acclimated for at least 2 weeks to the control
water quality and stocking conditions before being use in
experiments. Each aquarium had 10 fish. The tests were
conducted in twelve 40-L glass aquaria filled with 30-L of
test solution. The fish were not fed during temperature
acclimation (24 hours) and the 96-hours test period. The pH
was adjusted using NaOH or H2SO4.
The 96-hour LC50 values were determined for various pH (6.00
- 6.45) and temperatures (1.8 - 17.8 °C). The resulting LC50
values (603-2758 mg/L) expressed in ammonium sulfate
concentrations, have been converted from 128 - 585 mg/L TAN
given in the report.
Reliability: (2) valid with restrictions
limited documentation
23-JAN-2004 (69)
Type:
static
Species:
other: Barbus ambassis (freshwater Barb)
Exposure period: 48 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: = 545
Limit Test: no
Method:
GLP:
Test substance:
Remark:
Result:
Test condition:
Reliability:
94
other: see Test Condition
no
other TS: ammonium sulfate, not further specified
Cited as ppm (1 ppm was converted to 1 mg/L)
No mortality occurred in controls. dissolved oxygen in all
the tanks was above 5 mg/L and the pH varied from 7.5 to
7.6.
No mortality occurred within 96 hours test period at 400 ppm
ammonium sulfate. However, concentrations higher than these
were found to be toxic.
Fishes were kept in the laboratory at room temperature for a
fortnight. Eight to ten fishes measuring about 4.5 cm in
length were used in each container. Replicate 96-hour static
bioassays were conducted in fresh water using five different
concentrations and two controls were run simultaneously.
Observations were made at 24, 48, 72, and 96 hours after the
beginning of the test. Experiments were carried out at a
controlled temperature of 28 deg C +/- 1 deg C. During the
experiments, dissolved oxygen, pH, nitrite and nitrate were
determined using standard procedures. LC50 values were
estimated by plotting the percentage mortality at 24- and
48-hours for each concentration and reading the estimated
concentraiton lethal to 50% of the fish from the graph.
Year of study: not reported.
(2) valid with restrictions
limited documentation
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