AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Species:
Strain:
Sex:
Vehicle:
Doses:
Value:
rat
Wistar
male/female
water
full LD50 test: not reported, -screening test: 2000 mg/kg bw
(limit test)
> 2000 mg/kg bw
Method: other: according to TG 423
GLP:
no data
Test substance: other TS: ammonium sulfate, commercial grade
Remark:
Result:
The study was undertaken to validate a screening test for
the assessment of the acute oral toxicity with a view to
minimizing the use of animals in the acute toxicity testing.
Two laboratories cooperated in this study.
Approximative LD50 in males:
in laboratory A > 2000 mg/kg bw,
in laboratory B ca. 2000 mg/kg bw
Approximative LD50 in females:
in laboratory A > 2000 mg/kg bw,
in laboratory B ca. 2000 mg/kg bw.
Clinical signs, necropsy findings: not reported.
Test condition: The full LD50 test (according to the guidelines of Japan) was
not performed at this study.
For the screening study, male and female Wistar rats of 5-6
weeks of age were treated with the test substance after at
least 5 days of adaptation. The test substance was
administered orally in a single dose after fasting for 16
hours.
Observation of animals, including body weight changes,
mortality, gross lesion and behavioural and clinical
abnormality, were performed for 14 days after exposure, and
necropsy was carried out at the end of the test.
The experiments were performed simultaneously in two
different laboratories.
Three male and three female rats were each given a dose of
2000 mg/kg bw. If none of the animals died, further toxicity
tests were not performed and the chemical was regarded as
having an LD50 of greater than 2000 mg/kg bw. If some of the
animals died, a step 2 test was performed at the level of
200 mg/kg bw. If all animals survived in the step 2 test,
the chemical was considered to have an LD50 greater than 200
mg/kg bw. When some of the animals died, the step 3 test
with 20 mg/kg bw was performed. If all the animals died at
this dose level, it meant that he chemical had an LD50 of
less than 20 mg/kg bw. If one or two animals died at the
fixed dose level, the chemical was regarded as having an
LD50 near that dose level, and the approximate LD50 of the
chemical was indicated as ca. 20, 200 or 2000 mg/kg bw.
Year of study: not reported.
Reliability: (2) valid with restrictions
limited documentation, no further information on purity
Flag:
Critical study for SIDS endpoint
11-APR-2006 (113)
Type:
LD50
UNEP PUBLICATIONS 147