AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Dunaliella sp up to 17 days NOEC > 0.942
Test condition: Ammonium sulfate concentrations consisting of 0, 5, 10, 25,
50, 100, 150, and 200 ug atom NH4-N/L were placed in 1-Liter
flasks. A control contained 880 ug atom/L NO3-N. After
inoculation, duplicate cultures were incubated in a water bath
at 21-22 °C under continuous illumination. Growth was measured
at daily or half-daily intervals for up to 17-days. For
photosynthesis studies, 90-mL of seawater containing the above
concentrations of NH4-N were placed in 125 mL bottles.
Radioactive NaH14CO3 was added and the bottles were incubated
for 3 hours.
Reliability: (2) valid with restrictions
Incomplete documentation.
16-JUL-2004 (81)
Species:
other aquatic plant: Chondrus crispus Stackhouse (Irish Moss)
Endpoint:
other: photosynthesis
Unit: mg/l Analytical monitoring:
LOEC: = 46.4
Method:
GLP:
Test substance:
other: see Test Condition
no data
other TS: ammonium sulfate, not further specified
Remark:
Cultivated Chondrus crispus was used in N-NH4 uptake
experiments in the laboratory. An elevation of temperature
increased the apparent rate of uptake, especially up to 11
deg C. Uptake in the dark was found to be 83% of that in
the light. The apparent uptake decreased with increasing
internal N pool; rates were 26.5, 22.2 and 20.2 ug N g dry
wt-1 min-1 for internal N pools of 2.7, 3.5 and 4.6%,
respectively. Apparent uptake increased with the substrate
N concentration. The resulting curve has two components: an
active uptake and a diffusion component at high (more than
5000 ug N L-1) external N levels. Ks and V max were
calculated by deducting the diffusion component from the
uptake curve: these were 497 ug N L-1 and 14.4 ug N g dry
wt-1 min-1, respectively, and reflect a low substrate
affinity.
Result: A concentration of 10 mg N/L (about 46.4 mg (NH4)2SO4 / L)
reduced photosynthesis by 12.8% and as much as 30 mg N / L
reduced photosynthesis by 34.5%. No other effects were
observed.
Test condition: The effect of ammonium sulfate on photosynthesis was tested
by following the variations of pH and calculating the
corresponding carbon equilibrium as described by Hansson,
1973 (Deep Sea Res. 20, 461-478). N uptake rates were
calculated between 5 and 15 minutes into the experiment,
normalizing values with the dry weight.
Incubation times varied from 15 minutes (Michaelis-Menten
experiments) to 5 hours.
Reliability: (2) valid with restrictions
limited documentation
13-JUL-2004 (82)
Species:
120
other algae:Synechococcus cedrorum
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