AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
Reliability: (2) valid with restrictions
limited documentation, open application, no further
information on purity
Flag:
Critical study for SIDS endpoint
24-AUG-2005 (113)
Type:
Species:
Strain:
Sex:
Vehicle:
Doses:
Value:
Method:
GLP:
Test substance:
Remark:
Result:
LD50
mouse
other: ddy
male/female
other: water-acetone solution (no further detail reported)
2000 mg/kg bw (limit test)
> 2000 mg/kg bw
other: see Test Condition
no data
other TS: ammonium sulphate, commercial grade
The study was undertaken to validate a screening test for
the assessment of the acute dermal toxicity with a view to
minimizing the use of animals in the acute toxicity testing.
Two laboratories cooperated in this study.
Approximative LD50 in males:
in laboratory A > 2000 mg/kg bw,
in laboratory B > 2000 mg/kg bw.
Approximative LD50 in females:
in laboratory A > 2000 mg/kg bw,
in laboratory B > 2000 mg/kg bw.
Test condition:
158
Clinical signs, necropsy findings: not reported.
Male and female ddy mice of 5-6 weeks of age were treated
with the test substance after at least 5 days of adaptation.
Hair was first removed from an area of 1x2 cm2 on the back
with an electric hair clipper, and then the test substances
dissolved in acetone and water were applied in a single dose
to the skin surface of the clipped backs of the animals. The
application sites were not covered but the treated areas
were prevented from being licked by using a plastic collar
or by fixing the animals on a plastic plate, and all animals
were individually housed in stainless-steel cages.
Observation of animals, including body weight changes,
mortality, gross lesion and behavioural and clinical
abnormality, were performed for 14 days after exposure, and
necropsy was carried out at the end of the test.
The experiments were performed simultaneously in two
different laboratories.
Three male and three female mice were each given a dose of
2000 mg/kg bw. If none of the animals died, further toxicity
tests were not performed and the chemical was regarded as
having an LD50 of greater than 2000 mg/kg bw. If some of the
animals died, a step 2 test was performed at the level of
200 mg/kg bw. IF all animals survived in the step 2 test,
the chemical was considered to have an LD50 greater than 200
mg/kg bw. When some of the animals died, the step 3 test
with 20 mg/kg bw was performed. If all the animals died at
this dose level, it meant that he chemical had an LD50 of
less than 20 mg/kg bw. If one or two animals died at the
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